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Single electron tunneling in lipid bilayers as a novel sensing principle

Research Project

Project/Area Number 26620111
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionTohoku University

Principal Investigator

Hirano-Iwata Ayumi  東北大学, 医工学研究科, 准教授 (80339241)

Co-Investigator(Kenkyū-buntansha) NIWANO MICHIO  東北大学, 電気通信研究所, 教授 (20134075)
KIMURA YASUO  東北大学, 電気通信研究所, 准教授 (40312673)
YAMAMOTO HIDEAKI  東北大学, 学際科学フロンティア研究所, 助教 (10552036)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脂質二分子膜 / 単電子トンネリング / ナノデバイス / 単電子トランジスタ
Outline of Final Research Achievements

This study aims at realization of single-electron tunneling in lipid bilayers as a new sensing principle. We first examined incorporation of nanoparticles into lipid bilayers and found that only very small nanoparticles could be incorporated into self-standing bilayer lipid membranes. We next examined simulation on single-electron effects of aligned nanoparticles and revealed that observable single-electron effects would be possible even at room temperature. In addition, we have established fabrication procedures for silicon chips with wired metal electrodes. We would like to combine these techniques to realize single-electron tunneling in lipid bilayers and apply to highly sensitive biosensors to demonstrate a novel sensing principle.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (51 results)

All 2016 2015 2014 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (39 results) (of which Int'l Joint Research: 3 results,  Invited: 12 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Reconstitution of Human Ion Channels into Solvent-Free Lipid Bilayers Enhanced by Centrifugal Forces2016

    • Author(s)
      A. Hirano-Iwata, Y. Ishinari, M. Yoshida, S. Araki, D. Tadaki, R. Miyata, K. Ishibashi, H. Yamamoto, Y. Kimura, and M. Niwano
    • Journal Title

      Biophys. J.

      Volume: 110

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of interfacial chemical states on the performance of perovskite solar cells2016

    • Author(s)
      Teng Ma, Daisuke Tadaki, Masao Sakuraba, Shigeo Sato, Ayumi Hirano-Iwata and Michio Niwano
    • Journal Title

      J. Mater. Chem. A

      Volume: 4 Issue: 27 Pages: 4392-4397

    • DOI

      10.1039/c5ta03039k

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings2016

    • Author(s)
      Matsumura R, *Yamamoto H, Niwano M, Hirano-Iwata A
    • Journal Title

      Appl Phys Lett

      Volume: 108 Issue: 2 Pages: 023701-023701

    • DOI

      10.1063/1.4939629

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Interaction of plasma-generated water cluster ions with chemically-modified Si surfaces investigated by infrared absorption spectroscopy2016

    • Author(s)
      Ayumi Hirano-Iwata, Ryosuke Matsumura, Teng Ma, Yasuo Kimura, Michio Niwano, Kazuo Nishikawa
    • Journal Title

      AIP Advances

      Volume: 6 Issue: 3

    • DOI

      10.1063/1.4944902

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Micro- and Nano-Technologies for Lipid Bilayer-Based Ion-Channel Functional Assays2015

    • Author(s)
      Ayumi Hirano-Iwata, Yutaka Ishinari, Hideaki Yamamoto, and Michio Niwano
    • Journal Title

      Chemistry-An Asian Journal

      Volume: 掲載確定 Issue: 6 Pages: 1266-1274

    • DOI

      10.1002/asia.201403391

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular doping of regioregular poly(3-hexylthiophene) layers by 2,3,5,6-tetrafluoro-7,7,8,8-tetracyanoquinodimethane investigated by infrared spectroscopy and electrical measurements2015

    • Author(s)
      Daisuke Tadaki, Teng Ma, Jinyu Zhang, Shohei Iino, Ayumi Hirano-Iwata, Yasuo Kimura, and Michio Niwano
    • Journal Title

      Jpn. J. Appl. Phys

      Volume: 54 Issue: 9 Pages: 091602-091602

    • DOI

      10.7567/jjap.54.091602

    • NAID

      210000145652

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interference between Field Excitatory Postsynaptic Potentials and Simultaneously Recorded Chronoamperometric L-Glutamate Currents in Mouse Hippocampal Slices2014

    • Author(s)
      Ayumi Hirano-Iwata, Ryosuke Matsumura, Ryuta Tezuka, Michio Niwano, Tim V. P. Bliss, Masao Sugawara
    • Journal Title

      Electrochem. Commun.

      Volume: 45 Pages: 1-4

    • DOI

      10.1016/j.elecom.2014.04.016

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Real-time monitoring of extracellular L-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based L-glutamate sensor2014

    • Author(s)
      Yuki Ikegami, Shizuko Hozumi, Atsushi Shoji, Ayumi Hirano-Iwata, Tim Bliss and Masao Sugawara
    • Journal Title

      Sensing and BioSensing Research

      Volume: 2 Pages: 31-37

    • DOI

      10.1016/j.sbsr.2014.10.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] シリコン微細加工で創るイオンチャネルチップ2014

    • Author(s)
      平野愛弓,石成 裕,木村康男,庭野道夫
    • Journal Title

      表面科学

      Volume: 35 Pages: 438-442

    • NAID

      130004678726

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 微細加工イオンチャネルチップにおけるチャネル包埋促進2016

    • Author(s)
      平野愛弓,吉田美優,荒木 駿,山本英明,庭野道夫
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 二次元系原子膜と脂質分子膜の融合デバイスへ向けた要素技術開拓2016

    • Author(s)
      磯部亜紀子, 寺崎真志, 木村康男, 平野愛弓, 荻野俊郎
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 液中環境下における金ナノ粒子の脂質二重膜への導入評価2016

    • Author(s)
      坂口直駿, 仲山智明, 木村僚佑, 木村康男, 平野愛弓, 荻野俊郎
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] シランカップリング剤修飾SiN基板表面における脂質膜の流動性比較2016

    • Author(s)
      山浦大地, 仲山智明, 木村康男, 平野愛弓, 荻野俊郎
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 安定化脂質二分子膜イオンチャネルセンサの開発2016

    • Author(s)
      吉田美優,平野愛弓,山本英明,庭野道夫
    • Organizer
      平成27年度日本表面科学会東北・北海道支部学術講演会
    • Place of Presentation
      東北大学
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] ナノテーパー構造に基づく人工細胞膜の構築2016

    • Author(s)
      荒木 駿,平野愛弓,但木大介,石橋健一,山本英明,庭野道夫
    • Organizer
      平成27年度日本表面科学会東北・北海道支部学術講演会
    • Place of Presentation
      東北大学
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Improved process of fabricating silicon chips with micro-apertures for formation of mechanically stable bilayer lipid membranes2016

    • Author(s)
      D. Tadaki, A. Hirano-Iwata, K. Ishibashi, S. Araki, M. Yoshida, K. Arata, T. Ohori, H. Yamamoto, M. Niwano
    • Organizer
      The Joint Symposium of 10th International Symposium on Medical, Bio- and Nano-Electronics and 7th International Workshop on Nanostructures & Nanoelectronics
    • Place of Presentation
      東北大学
    • Year and Date
      2016-03-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微細加工シリコン基板を用いた人工細胞膜の構築2015

    • Author(s)
      荒木 駿, 平野愛弓, 山本英明, 庭野道夫
    • Organizer
      2015年真空・表面科学合同講演会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 金ナノ粒子を包埋した脂質二重膜の形態観察2015

    • Author(s)
      坂口直駿, 仲山智明, 木村僚祐, 木村康男, 平野愛弓, 荻野俊郎
    • Organizer
      2015年真空・表面科学合同講演会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Reconstitution of Ion Channel Functions in Microfabricated Silicon Chips2015

    • Author(s)
      Ayumi Hirano-Iwata
    • Organizer
      Joint Symposium of Sendai Symposium on Analytical Sciences 2015 and Tohoku Univ.-IMEC Seminar
    • Place of Presentation
      東北大学
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微細加工シリコン基板に基づくイオンチャネル機能の発現2015

    • Author(s)
      吉田美優,山本英明,平野愛弓,庭野道夫
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 液中環境下における金ナノ粒子の脂質二重膜への導入評価2015

    • Author(s)
      坂口 直駿,仲山 智明,木村 僚佑,木村 康男,平野 愛弓,荻野 俊郎
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] An ion-channel biosensor based on microfabrication2015

    • Author(s)
      平野愛弓
    • Organizer
      化学系学協会東北大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 微細加工シリコン基板に基づく脂質二分子膜イオンチャネルチップ2015

    • Author(s)
      平野愛弓
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 微細加工シリコン基板を用いたイオンチャネル機能の再構成2015

    • Author(s)
      平野愛弓
    • Organizer
      電子情報通信学会ソサイエティー大会
    • Place of Presentation
      東北大学
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 半導体微細加工で創る脂質二分子膜イオンチャネルチップ2015

    • Author(s)
      平野愛弓
    • Organizer
      物性研・短期研究会「反応と輸送」
    • Place of Presentation
      東京大学
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Au nanoparticle incorporation into supported lipid bilayer membranes and its characterization by atomic force microscopy2015

    • Author(s)
      Naotoshi Sakaguchi, Kenji Yokota, Tomoaki Nakayama, Ryosuke Kimura, Yasuo Kimura, Ayumi Hirano-Iwata, Toshio Ogino
    • Organizer
      Eighth International Conference on Molecular Electronics and Bioelectronics (M&BE8)
    • Place of Presentation
      Tower Hall Funabori, Tokyo
    • Year and Date
      2015-06-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 半導体微細加工に基づく脂質二分子膜イオンチャネルチップ2015

    • Author(s)
      平野愛弓
    • Organizer
      神奈川R&D推進協議会イノベーション研究部会技術討論会
    • Place of Presentation
      旭硝子中央研究所
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 微細加工シリコン基板に基づくイオンチャネル機能の再構成2015

    • Author(s)
      平野愛弓,山本英明,木村康男,庭野道夫
    • Organizer
      電子情報通信学会電子デバイス研究会
    • Place of Presentation
      東北大学
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] シリコン微細加工で創る脂質二分子膜イオンチャネルチップ2015

    • Author(s)
      平野愛弓
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学,平塚
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 規則ナノ細孔を有する多孔質薄膜を用いたナノバブル発生2015

    • Author(s)
      齋藤栄幸,馬 騰,山本英明,平野愛弓,庭野道夫
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学,平塚
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 溶液塗布法による有機強誘電体薄膜(PVDF薄膜)の成膜2015

    • Author(s)
      中山貴裕,今井裕司,馬 騰,山本英明,平野愛弓,庭野道夫
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学,平塚
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子を含む脂質二重膜の液中におけるAFM評価2015

    • Author(s)
      坂口直駿,横田圭司,仲山智明,木村僚佑,木村康男,平野愛弓,荻野俊郎
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学,平塚
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] ナノ構造設計に基づく脂質二分子膜イオンチャネルチップの開発2015

    • Author(s)
      平野愛弓
    • Organizer
      日本分析化学会東北支部受賞講演会
    • Place of Presentation
      東北大学,仙台
    • Year and Date
      2015-03-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] シリコン微細加工に基づく脂質二分子膜イオンチャネルチップの開発2015

    • Author(s)
      平野愛弓
    • Organizer
      日本学術振興会「薄膜第131委員会、半導体界面制御技術第154委員会」合同研究会
    • Place of Presentation
      ホテルアジュール竹芝,東京
    • Year and Date
      2015-02-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 微細加工シリコン基板とイオンチャネルの融合2014

    • Author(s)
      石成 裕,平野愛弓,山本英明,木村康男,庭野道夫
    • Organizer
      第34回表面科学学術講演会
    • Place of Presentation
      くにびきメッセ,松江
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Microfabricated Si Chips for Reconstitution of Ion Channel Proteins2014

    • Author(s)
      Ayumi Hirano-Iwata, Yutaka Ishinari, Hideaki Yamamoto, Yasuo Kimura, and Michio Niwano
    • Organizer
      The 7th International Symposium on Surface Science (ISSS-7)
    • Place of Presentation
      くにびきメッセ,松江
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Interference between chronoamperometric L-glutamate currents and simultaneously recorded field excitatory postsynaptic potentials2014

    • Author(s)
      R. Matsumura, A. Hirano-Iwata, H. Yamamoto, M. Niwano
    • Organizer
      The 7th International Symposium on Surface Science (ISSS-7)
    • Place of Presentation
      くにびきメッセ,松江
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Behavior of plasma-generated water cluster ions at chemically-modified Si surfaces investigated by surface infrared spectroscopy2014

    • Author(s)
      A. Hirano-Iwata, Y. Kimura, K. Okada, N. Yamashita, M. Teng, M. Niwano and K. Nishikawa
    • Organizer
      The 7th International Symposium on Surface Science (ISSS-7)
    • Place of Presentation
      くにびきメッセ,松江
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Infrared absorption spectroscopic study of surface chemical reactions on Pd exposed to hydrogen and oxygen2014

    • Author(s)
      T. Nakayama, Y. Imai, Y. Kimura, A. Hirano-Iwata, M. Teng1 and M. Niwano
    • Organizer
      The 7th International Symposium on Surface Science (ISSS-7)
    • Place of Presentation
      くにびきメッセ,松江
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Microfabricated Si Chips Containing human Ether-a-go-go-Related Gene Channels as a Platform for Drug Safety Screenings2014

    • Author(s)
      Ayumi Hirano-Iwata, Yutaka Ishinari, Yasuo Kimura, and Michio Niwano
    • Organizer
      2014 ECS and SMEQ Joint International Meeting
    • Place of Presentation
      Moon place resort hotel, Cancun, Mexico
    • Year and Date
      2014-10-05 – 2014-10-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Simultaneous Recording of Chronoamperometric L-Glutamate Current and Field Excitatory Postsynaptic Potentials in Mouse Hippocampal Slice2014

    • Author(s)
      R. Matsumura, A. Hirano-Iwata, M. Niwano, T. Bliss, M. Sugawara
    • Organizer
      2014 ECS and SMEQ Joint International Meeting
    • Place of Presentation
      Moon place resort hotel, Cancun, Mexico
    • Year and Date
      2014-10-05 – 2014-10-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Recording Ion-Channel Activities Based on Microfabricated Silicon Chips2014

    • Author(s)
      Yutaka Ishinari, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター,札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ナノデバイス構築のための局所陽極酸化セルフアラインメント技術2014

    • Author(s)
      木村康男,平野愛弓,庭野道夫
    • Organizer
      第75回応用物理学会秋季学術講演会
    • Place of Presentation
      北海道大学,札幌
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] グルタミン酸電流と興奮性シナプス後電位の同時測定2014

    • Author(s)
      松村亮佑,平野愛弓,山本英明,庭野道夫
    • Organizer
      第75回応用物理学会秋季学術講演会
    • Place of Presentation
      北海道大学,札幌
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス脳スライスにおけるグルタミン酸電流に及ぼす興奮性後シナプス電位の影響2014

    • Author(s)
      平野愛弓, 松村亮佑,菅原正雄,庭野道夫
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学,東広島
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] マイクロコンタクトプリンティング法による培養マウス海馬神経細胞の軸索誘導2014

    • Author(s)
      高沖 英里,山本 英明,桂林 秀太郎,木村 康男,平野 愛弓,庭野 道夫
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Bio-electroanalytical Methods Targeted for Neuronal Functions2014

    • Author(s)
      Ayumi Hirano-Iwata
    • Organizer
      International Workshop “Bioelectronics”
    • Place of Presentation
      Kleinwalsertal (Austria)
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 微細加工で創る脂質二分子膜イオンチャネルチップ2014

    • Author(s)
      平野 愛弓
    • Organizer
      (独)日本学術振興会「分子系の複合電子機能第181委員会」第19回研究会
    • Place of Presentation
      化学会館,東京
    • Year and Date
      2014-06-10 – 2014-06-11
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] すごいぞ!身の回りの表面科学(分担執筆)2015

    • Author(s)
      平野愛弓(他44名)
    • Total Pages
      270
    • Publisher
      講談社
    • Related Report
      2015 Annual Research Report
  • [Remarks] 平野研究室ホームページ

    • URL

      http://www.ecei.tohoku.ac.jp/hirano/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 平野研究室 ホームページ

    • URL

      http://www.ecei.tohoku.ac.jp/hirano/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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