Thermodynamic study of RNA trinucleotide recognition within silica mesopores
| Project/Area Number |
26620112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ナノ細孔 / 三塩基認識 / DNA / 3塩基認識 / ３塩基認識 |
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| Outline of Final Research Achievements |
In the present study, we aimed to clarify trinucleotide recognition process occurred inside meso-sized cavities of mesoporous silica. As the results, we found following three characteristics in the trinucleotide recognition: (1) efficient duplex formation of short (3- and 4-mer) DNA fragments could be achieved inside silica mesopores under cryogenic conditions, (2) DNA hairpin structure, on the other hand, was destabilized by the confinement inside the mesopores, (3) the duplex stability was significantly enhanced by size-matching between the pore size and the duplex width.
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Report
(4 results)
Research Products
(17 results)
