| Project/Area Number |
26620125
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
Nakanishi Jun 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 独立研究者 (60360608)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ナノ粒子 / がん / パッチー / 上皮成長因子 / ポリエチレングリコール / 分子込み合い / ラフト / 自己組織化 |
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| Outline of Final Research Achievements |
The purpose of the present study is to develop a method for the preparation of patchy nanoparticles, where ligand molecules are immobilized on the particle surface in a patchy fashion. We hypothesized that cells that abundantly express corresponding membrane receptors can be labeled with the patchy nanoparticles by precisely tuning the patchiness and molecular crowding of the immobilized ligands. As a proof-of-concept experiment, we functionalized gold nanoparticles with epidermal growth factor and photocleavable poly(ethylene glycol) (PEG). This particle exhibited photoirradiation- dependent increase in the interaction with the EGF receptor molecules. To further look into the interactions of EGF-immobilized gold nanoparticles with the cells, we isolated detergent-resistant membrane fraction on sucrose density gradients. We found the EGF receptors activated by the EGF-immobilized patchy particles exhibited distinct membrane trafficking from those of soluble EGF.
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