Development of optogenetic tools for controlling kinase activities in insulin signaling pathways and their practical applications
Project/Area Number |
26620128
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
Ozawa Takeaki 東京大学, 理学(系)研究科(研究院), 教授 (40302806)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 光制御 / インスリン / キナーゼ / リン酸化 / 酵素 / Akt / ERK |
Outline of Final Research Achievements |
We developed an optogenetic tools for controlling kinase activities of Akt and c-Raf, which are crucial proteins for insulin signaling. Akt was connected with a photo-receptor (CRY2). Upon irradiation with blue light, we observed movement of Foxo1 from nucleus to the cytosol. Moreover, we demonstrated quantitative control of Atrogin-1 gene expression by blue light. Using the same approach, an optogenetic technique to control c-Raf activity in living cells. We demonstrated that red light irradiation enabled to quantitative regulate c-Raf activity. The developed techniques will be useful tools for quantitative analysis of intracellular insulin signaling in living cells.
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Report
(3 results)
Research Products
(16 results)