| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We developed an optogenetic tools for controlling kinase activities of Akt and c-Raf, which are crucial proteins for insulin signaling. Akt was connected with a photo-receptor (CRY2). Upon irradiation with blue light, we observed movement of Foxo1 from nucleus to the cytosol. Moreover, we demonstrated quantitative control of Atrogin-1 gene expression by blue light. Using the same approach, an optogenetic technique to control c-Raf activity in living cells. We demonstrated that red light irradiation enabled to quantitative regulate c-Raf activity. The developed techniques will be useful tools for quantitative analysis of intracellular insulin signaling in living cells.
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