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Development of molecular probes for Raman bioimaging

Research Project

Project/Area Number 26620129
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

OKAMOTO Akimitsu  東京大学, 先端科学技術研究センター, 教授 (60314233)

Project Period (FY) 2014-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywordsラマン分光 / 炭素-炭素三重結合 / 細胞イメージング / 刺激応答性 / 炭素-炭素三重結合
Outline of Final Research Achievements

The objective of this research is to develop new labeling molecules for Raman bioimaging. Raman spectroscopy is one of the major candidates for gaining the multimodality of bioimaging. We chemically synthesized new labeling agents showing a specific wave number at 2000cm-1, which is not cell-characteristic wave number. These labeling molecules exhibited the Raman signals only when the molecules are activated with external stimulation under a mild condition. Finally, we observed molecular behavior in cells after we labeled the cells with the functional Raman labels tethered to the target biomolecules.

Report

(2 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (5 results)

All 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) (of which Invited: 2 results)

  • [Journal Article] FTO-Dependent Demethylation of N6-Methyladenosine Regulates mRNA Splicing and Is Required for Adipogenesis2014

    • Author(s)
      Yang, Y. G.; Zhao, X.; Yang, Y.; Sun, B. F.; Shi, Y.; Yang, X.; Xiao, W.; Hao, Y.; Ping, X.-L.; Chen, Y.; Wang, W.-J.; Jin, K.-X.; Wang, X.; Huang, C.; Fu, Y.; Ge, X.; Song, S.; Jeong, H. S.; Yanagisawa, H.; Niu, Y.; Jia, G.; Wu, W.; Tong, W.-M.; Okamoto, A.; He, C.; Danielsen, J. M.; Wang, X.
    • Journal Title

      Cell Res.

      Volume: 24 Pages: 1403-1419

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of Nucleic Acids with Synthetic Excitation-Controlled Fluorescent Oligonucleotide Probes2014

    • Author(s)
      Wang, D. O.; Okamoto, A.
    • Journal Title

      Methods Mol. Biol.

      Volume: 1262 Pages: 69-87

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA-Osmium Complexes: Recent Developments for Operative Chemical Analysis of DNA Epigenetic Modification2014

    • Author(s)
      Okamoto, A.
    • Journal Title

      ChemMedChem

      Volume: 9 Pages: 1958-1965

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Chemical Epigenetics: Chemical Visualization of Methylated DNA and Expressed RNA2014

    • Author(s)
      Akimitsu Okamoto
    • Organizer
      ConTech2014 RCAST-AICT Joint Symposium
    • Place of Presentation
      Suwon, Korea
    • Year and Date
      2014-11-28 – 2014-11-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] DNA修飾検出に対するいくつかの化学的アプローチ2014

    • Author(s)
      岡本 晃充
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2014-04-04   Modified: 2016-06-03  

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