| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Protein supramolecular architectures, such as cages, rings, tubes and two-dimensional arrays, have attracted a great deal of interest in bio-nanotechnology and material sciences. In this research, we demonstrated novel method to construct protein supramolecular architectures using protein crystals, which have highly ordered 3D arrangements of protein molecules. We used polyhedra crystals, in vivo protein crystals to construct nanoacup structures. We designed polyhedra mutants, in which cysteine residues were introduced on the interfaces of monomer to form disulfide bonds within the crystals, for construction of nanocap structures. We succeeded in construction of the nanocap structures by oxidation and dissolution of the crystals. The nanocup structures were characterized by X-ray structure analysis, MALDI TOF-MS and SDS-PAGE. This method can be applied to various protein crystals to construct protein architectures.
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