Construction of Protein Supramolecular Architectures by Etching of Protein Crystals

• Project/Area Number: 26620130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: ABE Satoshi 東京工業大学, 生命理工学研究科, 助教 (40508595)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: タンパク質結晶 / 多角体 / 超分子構造体 / ジスルフィド結合 / 結晶エンジニアリング / 超分子タンパク質 / 細胞内結晶

Outline of Final Research Achievements

Protein supramolecular architectures, such as cages, rings, tubes and two-dimensional arrays, have attracted a great deal of interest in bio-nanotechnology and material sciences. In this research, we demonstrated novel method to construct protein supramolecular architectures using protein crystals, which have highly ordered 3D arrangements of protein molecules. We used polyhedra crystals, in vivo protein crystals to construct nanoacup structures. We designed polyhedra mutants, in which cysteine residues were introduced on the interfaces of monomer to form disulfide bonds within the crystals, for construction of nanocap structures. We succeeded in construction of the nanocap structures by oxidation and dissolution of the crystals. The nanocup structures were characterized by X-ray structure analysis, MALDI TOF-MS and SDS-PAGE. This method can be applied to various protein crystals to construct protein architectures.

Research Products

• [Journal Article] Design of a Confined Environment using a Protein Cage and Crystals in Development of Biohybrid Materials (2016)
  • Author(s): S. Abe, B. Maity, and T. Ueno
  • Journal Title: Chem. Commun. Volume: 52 Issue: 39 Pages: 6496-6512
  • DOI: 10.1039/c6cc01355d
  • NAID: 120006582498
  • Peer Reviewed / Open Access
• [Journal Article] Design of Enzyme-Encapsulated Protein Containers by in Vivo Crystal Engineering (2015)
  • Author(s): Abe, S., Ijiri, H., Negishi, H., Yamanaka, H., Sasaki, K., Hirata, K., Mori, H., Ueno, T.
  • Journal Title: Adv. Mater. Volume: 27 Issue: 48 Pages: 7951-7956
  • DOI: 10.1002/adma.201503827
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Design of Protein Crystals in the Development of Solid Biomaterials (2015)
  • Author(s): S. Abe and T. Ueno
  • Journal Title: RSC Advances Volume: ５ Issue: 27 Pages: 21366-21375
  • DOI: 10.1039/c4ra16748a
  • Peer Reviewed
• [Journal Article] Surface Functionalization of Protein Crystals with Carbohydrate Using Site-selective Bioconjugation (2015)
  • Author(s): S. Abe, Y. Tokura, R. Pal, N. Komura, A. Imamura, K. Matsumoto, H. Ijiri, N. J. M. Sanghamitra, H. Tabe, H. Ando, M. Kiso, H. Mori, S. Kitagawa, and T. Ueno
  • Journal Title: Chem. Lett. Volume: 44 Issue: 1 Pages: 29-31
  • DOI: 10.1246/cl.140865
  • NAID: 130004693848
  • Peer Reviewed
• [Journal Article] Preparation of a Cross-linked Porous Protein Crystal Containing Ru Carbonyl Complexes as a CO-releasing Material (2015)
  • Author(s): Hiroyasu Tabe, Kenta Fujita, Satoshi Abe, Masahiko Tsujimoto, Takahiro Kuchimaru, Shinae Kizana-Kondoh, M. Takano, Susumu Kitagawa and Takafumi Ueno
  • Journal Title: Inorganic Chemistry Volume: 54 Issue: 1 Pages: 215-220
  • DOI: 10.1021/ic502159x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] タンパク質結晶の分子設計によるバイオ固体材料の開発 (2015)
  • Author(s): 安部 聡、上野隆史
  • Journal Title: 化学工業 Volume: 66 Pages: 264-272
• [Presentation] In vivo Crystal Engineering for Protein Porous Materials (2016)
  • Author(s): 安部 聡、森 肇、上野隆史
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-25
• [Presentation] 細胞内結晶化を利用したタンパク質固体材料の開発 (2015)
  • Author(s): 安部 聡
  • Organizer: 第154回 東海高分子研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] 細胞内結晶工学によるタンパク質結晶のナノ空間設計 (2015)
  • Author(s): 安部 聡、森 肇、上野隆史
  • Organizer: 第9回 バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-10
• [Presentation] タンパク質集合体を用いた有機金属酵素の構築 (2015)
  • Author(s): 安部 聡
  • Organizer: 第28回 生物無機化学夏季セミナー
  • Place of Presentation: 伊東
  • Year and Date: 2015-08-28
  • Invited
• [Presentation] 蛋白質結晶細孔設計による機能性分子の集積制御 (2015)
  • Author(s): 安部 聡、田部博康、赤井祐貴、森 肇、北川進、上野隆史
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 機能性タンパク質集合体の構築 (2014)
  • Author(s): 安部 聡
  • Organizer: 第1回「動的秩序と機能：」若手研究会
  • Place of Presentation: 宮城
  • Year and Date: 2014-09-28 – 2014-09-30
• [Presentation] 蛋白質結晶内分子設計による超分子構造体の構築 (2014)
  • Author(s): 安部 聡、根岸 走、上野隆史
  • Organizer: 第8回バイオ関連化学シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 細胞内タンパク質結晶の形成機構の解明 (2014)
  • Author(s): 安部 聡、徳増沙耶、森 肇、上野隆史
  • Organizer: 第63回高分子学会年次大会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-28 – 2014-05-30