Regulation of nitrous oxide formation in cell
Project/Area Number |
26620140
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Yoshitsugu Shiro 国立研究開発法人理化学研究所, 放射光科学総合研究センター, 主任研究員 (70183051)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 一酸化窒素 / 亜酸化窒素 / 脱窒菌 / 嫌気呼吸 / 温室効果ガス / オゾン層破壊物質 / 細胞内タンパク質複合体 |
Outline of Final Research Achievements |
Nitrous oxide (N2O) is not only a green-house gas but also ozone-depleting substance. About 70% of the global emission of N2O is attributable to the anaerobic respiration of denitrification micro-organisms. We aimed to elucidate and to regulate a molecular mechanism of the N2O generation by the micro-organisms. On he basis of the X-ray crystallographic analysis, the functional analysis of some mutants and the molecular dynamic simulation, we proposed that the complex of the NO generation enzyme (nitrite reductase) and NO detoxifying enzyme (nitric oxide reductes) in micro-organism cell is a key player for the N2O generation. We also found four compounds which inhibit the NiR function, and eventually the N2O formation.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Dynamics of Nitric Oxide Controlled by Protein Complex in Bacterial System2017
Author(s)
E. Terasakaa, K. Yamada, P.-H. Wang, K. Hosokawa, R. Yamagiwa, K. Matsumoto, S. Ishii, T. Mori, K. Yagi, H. Sawai, H. Arai, H. Sugimotoa, Y. Sugitac, Y. Shiroa, T. Tosha
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Journal Title
Proc. Natl. Acd. SCI. USA
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Crystal structure of bacterial haem importer complex in the inward-facing conformation2016
Author(s)
Naoe, Y., Nakamura, N., Doi, A., Sawabe, M., Nakamura, H., Shiro, Y., Sugimoto, H.
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Journal Title
Nature Communications
Volume: 7
Issue: 1
Pages: 13411-13411
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A Substrate-Binding-State Mimic of H2O2-Dependent Cytochrome P450 Produced by One-point Mutagenesis and Peroxygenation of Non-native Substrates2016
Author(s)
O. Shoji, T. Fujishiro, K. Nishio, Y. Kano, H. Kimoto, S. Chien, H. Onoda, A. Muramatsu, S. Tanaka, A. Hori, H. Sugimoto, Y. Shiro, Y. Watanabe
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Journal Title
Catal. Sci. Tech.
Volume: 6
Issue: 15
Pages: 5806-5811
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] A Nearly On-axis Spectroscopic System for Simultaneously Measuring UV-visible Absorption and X-ray Diffraction in the SPring-8 Structural Genomics Beamline2016
Author(s)
M. Sakaguchi, T. Kimura, T. Nishida, T. Tosha, Y. Yamaguchi, S. Yanagisawa, G. Ueno, H. Murakami, H. Ago, M. Yamamoto, T. Ogura, Y. Shiro, M. Kubo
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Journal Title
J. Synchro. Rad.
Volume: 23
Issue: 1
Pages: 334-338
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Palladium-Nanoparticle-Catalyzed 1,7-Palladium Migration Involving C-H Activation, Followed by Intramolecular Amination: Regioselective Synthesis of N1-Arylbenzotriazoles and an Evaluation of Their Inhibitory Activity Towards Indoleamine 2,3-Dioxygenase2014
Author(s)
K. Takagi, M. Al-Amin, N. Hoshiya, J. Wouters, H. Sugimoto, Y. Shiro, H. Fukuda, S. Shuto, M. Arisawa
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Journal Title
J. Org. Chem.
Volume: 79
Pages: 6366-6371
Related Report
Peer Reviewed
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[Journal Article] Structure-function Analyses of Cytochrome P450revI Involved in Reveromycin A Biosynthesis and Evaluation of the Biological Activity of Its Aubstrate, Reveromycin T2014
Author(s)
S. Takahashi, S. Nagano, T. Nogawa, N. Kanoh, M. Uramoto, M. Kawatani, T. Shimizu, T. Miyazawa, Y. Shiro, H. Osada
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Journal Title
J. Biol. Chem.
Volume: 289
Pages: 32446-32458
Related Report
Peer Reviewed
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[Presentation] Damage-free X-ray Crystallographic Analysis of P450nor in the NO Bound Form using SACLA.2016
Author(s)
Kubo, M., Nomura, T., Nishida, T., Tosha, T., Sugimoto, H., Kimura, T., Takano, Y., Yamashita, K., Hirata, K., Ueno, G., Ago, H., Yamamoto, M., Shiro, Y.
Organizer
British Biophysical Society 2016 Biennial Meeting
Place of Presentation
Liverpool, UK
Year and Date
2016-07-07
Related Report
Invited
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[Book] 鉄の事典2014
Author(s)
藤井 浩、城 宜嗣
Total Pages
820
Publisher
朝倉書店
Related Report
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