| Project/Area Number |
26620141
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shirota Koichiro 国立研究開発法人理化学研究所, 小林脂質生物学研究室, 先任研究員 (00291071)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂質 / リポソーム / ラマン分光 / 細胞膜 / 光ピンセット / 表面増強ラマン |
|---|
| Outline of Final Research Achievements |
Lipid molecules of cell membranes are different in the inner and outer layers, and form domains in which the specific lipid molecules aggregate. In particular, the domains on the plasma membrane made of sphingolipid and cholesterol, which are termed lipid rafts, are drawing attention. Sphingomyelin (SM), which is typical "raft lipids", has both donor and acceptor of hydrogen bonds in the molecule, and can form hydrogen bonds between the molecules. Since Raman spectroscopy is a powerful analysis method for detecting intermolecular interactions such as hydrogen bonding, we applied it to determine the membrane distribution of SM molecules.
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