Discovery and application of frequency dependence of microwave-assisted degradation of mucin

Research Project

Project/Area Number	26620142
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Bio-related chemistry
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	KAMEYAMA AKIHIKO 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 上級主任研究員 (80415661)
Co-Investigator(Kenkyū-buntansha)	杉山順一国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (10235905) 清水弘樹国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30344716)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	ムチン / ヒアルロン酸 / マイクロ波 / 糖ペプチド
Outline of Final Research Achievements	We have studied a microwave-assisted degradation of mucins that have bound water molecules. MUC2 tandem repeat of 16 mer peptide containing 8 GalNAc residues has been synthesized as a model compound. As a model compound for degradation experiments, we used hyaluronic acid which should have bound water molecule as with mucins. For monitoring degradation of hyaluronic acid, we have developed affinity supported molecular matrix electrophoresis using 1%polyethyleneimine in polyvinyl alcohol. We have revealed that microwave-assisted heating of hyaluronic acid was dielectric heat in higher frequency than 1 to 2 GHz, while it was induction heat in lower frequency.