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Development of evaluation method for hiPSC-derived neuron function using nanofiber devices-based neuron chip

Research Project

Project/Area Number 26630096
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionKyoto University

Principal Investigator

Chen Yong  京都大学, 物質-細胞統合システム拠点, 教授 (70512458)

Co-Investigator(Kenkyū-buntansha) LIU Li  京都大学, 物質-細胞統合システム拠点, 助教 (50380093)
AIBA Kazuhiro  京都大学, 物質-細胞統合システム拠点, 準教授 (30564752)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords神経細胞 / ナノファイバー / 薬剤評価
Outline of Final Research Achievements

In vitro study of human induced pluripotent stem cells (hiPSCs)-derived neuron has great importance for biomedical research and for understanding of information processing of central and peripheral nervous systems. In this study, hiPSCs are differentiated toward neurons by using a culture patch devices made of crosslinked monolayer gelatin nanofibers. Comparing to the conventional culture dish method, the nanofiber devices method is more effective for culture and differentiation of stem cells. Then, neurons were obtained and used directly for electrophysiological measurement with a commercial MEA device, showing characteristic neuron activities. Thus, the patch method is advantageous over the conventional dish method for both motor neuron differentiation and electrophysiological measurements, providing a novel and powerful way for neuron based assays for the benefit of plug-and-play users.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2016 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results)

  • [Int'l Joint Research] Ecole Normale Superieure, Paris(France)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers2016

    • Author(s)
      Yadong Tang, Li Liu, Junjun Li, Leqian Yu, Francesco Paolo Ullo Severino, Li Wang, Jian Shi, Xiaolong Tu, Vincent Torre, Yong Chen
    • Journal Title

      Journal of Materials Chemistry B

      Volume: - Issue: 19 Pages: 3305-3312

    • DOI

      10.1039/c6tb00351f

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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