Hyper-miniaturization of fluorescence multisensor for pathogens with high sensitivity by evanescent light
Project/Area Number |
26630243
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Civil and environmental engineering
|
Research Institution | Hokkaido University |
Principal Investigator |
Satoh Hisashi 北海道大学, 工学(系)研究科(研究院), 准教授 (80326636)
|
Co-Investigator(Kenkyū-buntansha) |
ISHII Satoshi 北海道大学, 大学院工学研究院, 助教 (10612674)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | バイオセンサ / 環境分析 / 衛生 / マイクロ・ナノデバイス |
Outline of Final Research Achievements |
Gold thin film was immobilized on a glass plate. DSP molecules, which served as self-assembled monolayer, were immobilized on it. Anti-E. coli O157 antibody was immobilized on it. The glass plate was called as a sensor chip. The electrical signal was measured through a redox couple [Fe(CN)6]3-/4- by cyclic voltammetric analysis. The lower detection limit was 10 cell/mL.
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Report
(3 results)
Research Products
(3 results)