| Project/Area Number |
26630244
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Civil and environmental engineering
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
Omura Tatsuo 東北大学, 未来科学技術共同研究センター, 教授 (30111248)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
DAISUKE SANO 北海道大学, 大学院工学研究院, 准教授 (80550368)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YOSHIFUMI MASAGO 国際連合大学, サステイナビリティ高等研究所, 研究員 (50507895)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 腸管系ウイルス / 細胞応答 / 迅速検出 / 感染性 / 下水処理水 / 組織細胞 / 自然免疫 |
|---|
| Outline of Final Research Achievements |
This study aimed to identify candidate genes for detecting infectious viruses when concentrated water samples were inoculated to cultivated human cell lines. A vaccine strain of poliovirus type 1 (PV1) was used as a test virus, and the INT407 cell line derived from human intestinal epithelial cells was used as a host cell line. The expression level of KCNJ4 and SCN7A genes were monitored in the post-infection of PV1. When PV1 was inoculated at multiplicity of infection (MOI) = 1, the expression levels of KCNJ4 and SCN7A genes were significantly increased compared to those in mock cells. These results indicate that these genes of ion-chancel proteins are available as cellular genetic markers for detecting the presence of infectious viruses in water samples.
|
