Rapid detection of infectious enteric viruses in water using cellular responses to virus infection

• Project/Area Number: 26630244
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Tohoku University
• Principal Investigator: Omura Tatsuo 東北大学, 未来科学技術共同研究センター, 教授 (30111248)
• Co-Investigator(Kenkyū-buntansha): DAISUKE SANO 北海道大学, 大学院工学研究院, 准教授 (80550368)
• Co-Investigator(Renkei-kenkyūsha): YOSHIFUMI MASAGO 国際連合大学, サステイナビリティ高等研究所, 研究員 (50507895)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腸管系ウイルス / 細胞応答 / 迅速検出 / 感染性 / 下水処理水 / 組織細胞 / 自然免疫

Outline of Final Research Achievements

This study aimed to identify candidate genes for detecting infectious viruses when concentrated water samples were inoculated to cultivated human cell lines. A vaccine strain of poliovirus type 1 (PV1) was used as a test virus, and the INT407 cell line derived from human intestinal epithelial cells was used as a host cell line. The expression level of KCNJ4 and SCN7A genes were monitored in the post-infection of PV1. When PV1 was inoculated at multiplicity of infection (MOI) = 1, the expression levels of KCNJ4 and SCN7A genes were significantly increased compared to those in mock cells. These results indicate that these genes of ion-chancel proteins are available as cellular genetic markers for detecting the presence of infectious viruses in water samples.

Research Products

• [Journal Article] Estimation of contamination sources of human enteroviruses in a wastewater treatment and reclamation system by PCR-DGGE (2014)
  • Author(s): Zheng Ji, Xiaochang C. Wang, Limei Xu, Chongmiao Zhang, Naoyuki Funamizu, Satoshi Okabe, Daisuke Sano
  • Journal Title: Food and Environmental Virology Volume: 6(2) Issue: 2 Pages: 99-109
  • DOI: 10.1007/s12560-014-9140-x
  • Peer Reviewed
• [Presentation] ヒト細胞由来遺伝子マーカーを用いた感染性ウイルスの迅速検出 (2016)
  • Author(s): 稲葉愛美、伊藤寿宏、大村達夫、岡部聡、佐野大輔
  • Organizer: 第50回日本水環境学会年会
  • Place of Presentation: アスティとくしま（徳島県徳島市）
  • Year and Date: 2016-03-16
• [Presentation] セライトを用いた下水流入水中のウイルス濃縮法の改善 (2015)
  • Author(s): 稲葉愛美，真砂佳史，大村達夫
  • Organizer: 第49回日本水環境学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ノロウイルスGI，GII群を対象とした定量PCR法の比較 (2014)
  • Author(s): 真砂佳史，風間しのぶ，当广謙太郎，斉藤繭子，今川稔文，鈴木陽，押谷仁，大村 達夫
  • Organizer: 第17回水環境学会シンポジウム
  • Place of Presentation: 滋賀県立大学（滋賀県彦根市）
  • Year and Date: 2014-09-08 – 2014-09-09
• [Presentation] 下水モニタリングと感染性胃腸炎サーベイランスによるノロウイルス流行状況調査 (2014)
  • Author(s): 風間しのぶ，真砂佳史，当广謙太郎，相馬奈央，今川稔文，鈴木陽，Xiaofang Liu，斉藤繭子，押谷仁，大村 達夫
  • Organizer: 第17回水環境学会シンポジウム
  • Place of Presentation: 滋賀県立大学（滋賀県彦根市）
  • Year and Date: 2014-09-08 – 2014-09-09
• [Presentation] Detection and genotyping of Norovirus from gastroenteritis surveillance and wastewater monitoring in 2012-2013 winter (2014)
  • Author(s): Kazama, S., Y. Masago, K. Tohma, N. Souma, T. Imagawa, A. Suzuki, X. Liu, M. Saito, H. Oshitani and T. Omura.
  • Organizer: 4th International Conference on Food and Environmental Virology
  • Place of Presentation: Ionian Academy, Corfu, Greece
  • Year and Date: 2014-09-02 – 2014-09-05