Identification of mushroom gene involving in absorption and concentration of Cs, and development of innovative technology by the exploitation
| Project/Area Number |
26630249
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Kagoshima National College of Technology |
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Principal Investigator |
Masahito Yamauchi 鹿児島工業高等専門学校, 都市環境デザイン工学科, 教授 (40239843)
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| Co-Investigator(Kenkyū-buntansha) |
重松 亨 新潟薬科大学, 応用生命科学部, 教授 (10315286)
井口 晃徳 新潟薬科大学, 応用生命科学部, 助教 (60599786)
山田 真義 鹿児島工業高等専門学校, 都市環境デザイン工学科, 准教授 (80469593)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Takashi 長岡技術科学大学, 工学研究科, 教授 (10280447)
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| Research Collaborator |
YAGI Fumio 鹿児島大学, 名誉教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | きのこ / セシウム / カリウム / カリウムトランスポーター / ビール粕 / 活性化剤 / 環境修復技術 / 菌類 / ビール粕培地 |
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| Outline of Final Research Achievements |
Primers of K transporters (TRKH) 1~4 and formyltetrahydrofolate synthetase with Cs binding site were designed using genome information on Pleurotus osteatus. PCR with extracted DNA and primers revealed TRKH 2
and 3 could be amplified. Real time RT-PCR on TRKH2 showed diazoxide (previously confirmed as activator for Cs intake) increased the gene expression, suggesting that this transporter was involved in the Cs intake.
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Report
(4 results)
Research Products
(5 results)
