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Deciphering the supporter microbes that facilitate syntrophic degradation in methanogenic ecosystem

Research Project

Project/Area Number 26630250
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Narihiro Takashi  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20421844)

Co-Investigator(Renkei-kenkyūsha) TAMAKI Hideyuki  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (00421842)
KAMAGATA Yoichi  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 研究部門付 (70356814)
Research Collaborator LIU Wen-Tso  イリノイ大学, 教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords微生物ゲノム / 嫌気共生細菌 / メタン生成菌 / シントロフィー / 環境動態 / メタン生成アーキア / 微生物 / 廃水処理 / 微生物生態
Outline of Final Research Achievements

In order to identify the metabolic function that promotes decomposition of organic substrates (e.g., propionate and butyrate) by "syntrophy" which is knows as a microbial interaction between syntrophic substrate metabolizers (syntrophs) and methanogenic archaea (methanogens), we aimed to elucidate the metabolic function of syntrophs and methanogens from their genomic information. As a result, we successfully identify the dominant microbial constituents that inhabit the syntrophy-associated methanogenic microbiota. The genomes of three butyrate-oxidizing syntrophs encode genes involved in degradation pathway of butyrate and biological energy conservation systems that require for the degradation. In conclusion, these metabolic functions contribute to the stabilization and promotion of biodegradation in anaerobic ecosystem.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Int'l Joint Research] イリノイ大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] イリノイ大学(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Genomics and Metagenomics in Microbial Ecology: Recent Advances and Challenges2017

    • Author(s)
      Narihiro T, Kamagata Y
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 1 Pages: 1-4

    • DOI

      10.1264/jsme2.ME3201rh

    • NAID

      130005519598

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] Comparative Genomics of Syntrophic Branched-Chain Fatty Acid Degrading Bacteria2016

    • Author(s)
      Narihiro T, Nobu MK, Tamaki H, Kamagata Y, Sekiguchi Y, Liu WT
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 3 Pages: 288-292

    • DOI

      10.1264/jsme2.ME16057

    • NAID

      130005265098

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Draft Genome Sequences of Methanoculleus horonobensis Strain JCM 15517, Methanoculleus thermophilus Strain DSM 2373, and Methanofollis ethanolicus Strain JCM 15103, Hydrogenotrophic Methanogens Belonging to the Family Methanomicrobiaceae.2016

    • Author(s)
      Narihiro T, Kusada H, Yoneda Y, Tamaki H.
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 2

    • DOI

      10.1128/genomea.00199-16

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Draft Genome Sequence of Syntrophomonas wolfei subsp. methylbutyratica Strain 4J5T (JCM 14075), a Mesophilic Butyrate- and 2-Methylbutyrate-Degrading Syntroph.2016

    • Author(s)
      Narihiro T, Nobu MK, Tamaki H, Kamagata Y, Liu WT.
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 2

    • DOI

      10.1128/genomea.00047-16

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The nexus of syntrophy-associated microbiota in anaerobic digestion revealed by long-term enrichment and community survey2015

    • Author(s)
      Narihiro, T., M.K. Nobu, N-K. Kim, Y. Kamagata, and W-T. Liu
    • Journal Title

      Environmental Microbiology

      Volume: 17 Issue: 5 Pages: 1707-1720

    • DOI

      10.1111/1462-2920.12616

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Comparative genomics of branched-chain fatty acid degrading syntrophs reveals multiple genes involved in β-oxidation and energy conservation system2016

    • Author(s)
      Narihiro T, Nobu MK, Tamaki H, Kamagata Y, Sekiguchi Y, Liu WT
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      モントリオール国際会議場, モントリオール, カナダ
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome analysis of branched chain fatty acid degrading syntrophs: “Syntrophomonas wolfei subsp. methylbutyratica” strain JCM 14075T and Syntrophothermus lipocalidus strain TGB-C1T.2015

    • Author(s)
      Narihiro T, Nobu MK, Tamaki H, Kamagata Y, Liu WT.
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      亀城プラザ(土浦市)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] エコゲノミクスによる嫌気性廃水処理プロセスに生息する未培養微生物群の機能解明2015

    • Author(s)
      成廣 隆, Masaru K. Nobu, 鎌形洋一, Wen-Tso Liu
    • Organizer
      第14回 LS-BT合同研究発表会
    • Place of Presentation
      産業技術総合研究所つくばセンター
    • Year and Date
      2015-02-03 – 2015-02-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of microbial communities in syntrophy-associated enrichments and anaerobic wastewater treatment processes2014

    • Author(s)
      Takashi Narihiro, Masaru K. Nobu, Nakyung Kim, Yoichi Kamagata, and Wen-Tso Liu
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2014-08-04 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Book] Anaerobic Cultivation (In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition)2015

    • Author(s)
      Narihiro T, Kamagata Y
    • Total Pages
      12
    • Publisher
      ASM Press, Washington, DC.
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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