| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Soluble methane monooxygenases (sMMO) are nonheme diiron enzymes that catalyze oxidation of various organic compounds. Diiron centers of sMMO are coordinated by two imidazole donors of His residues and by four carboxylate donors of Glu residues. In this study we synthesized four different carboxylate-rich dinucleating ligands, 1,2-bis(N-benzyl-2-amino- methyl-6-pyridyl)ethane-N,N’-diglycine (H2Lgly), 1,2-bis(6-aminomethyl-2-pyridyl)ethane-N,N’-di- (norleucine) (H2Lnle), 1,2-bis(6-aminomethyl-2-pyridyl)ethane-N,N’-di(N-methylnorleucine) (H2LMenle), 1,2-bis(N-(9-anthracene)-2-aminomethyl-6-pyridyl)ethane (H2Lanthracenegly). These ligands form diiron complexes, which rapidly and efficiently catalyzed the oxidation of alkanes with m-CPBA as an oxidant to show high A/K ratio, large turnover number, large turnover frequency in the catalytic oxidations. These are attained by the carboxylate-rich coordination environment.
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