Sequential enzyme complex formation on DNA
Project/Area Number |
26630419
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
|
Research Institution | The University of Tokyo |
Principal Investigator |
Hirakawa Hidehiko 東京大学, 工学(系)研究科(研究院), 助教 (90451799)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 酵素複合体 / 核内増殖抗原 / 古細菌 / Metallosphaera sedula / DNA / リガーゼ |
Outline of Final Research Achievements |
We tried to develop a method that arrange the order of multiple enzymes on DNA to construct artificial multienzyme complexes that catalyze sophisticated sequential or concerted reactions. To retain enzymes on DNA, we focused on a DNA sliding clamp. A stable DNA sliding clamp was discovered from a archaeon and then characterized. A DNA-binding protein was immobilized on DNA at the starting point of complex formation to avoid drop-off of proteins fused to the DNA sliding clamp. As as result, we successfully constructed multienzyme complexes on DNA.
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Report
(3 results)
Research Products
(9 results)