| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We tried to develop a method that arrange the order of multiple enzymes on DNA to construct artificial multienzyme complexes that catalyze sophisticated sequential or concerted reactions. To retain enzymes on DNA, we focused on a DNA sliding clamp. A stable DNA sliding clamp was discovered from a archaeon and then characterized. A DNA-binding protein was immobilized on DNA at the starting point of complex formation to avoid drop-off of proteins fused to the DNA sliding clamp. As as result, we successfully constructed multienzyme complexes on DNA.
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