In vitro fabrication of bone-like structures for tissue engineering applications
| Project/Area Number |
26630423
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Yokohama National University |
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Principal Investigator |
Fukuda Junji 横浜国立大学, 工学(系)研究科(研究院), 准教授 (80431675)
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| Co-Investigator(Kenkyū-buntansha) |
ANADA TAKAHISA 東北大学, 歯学研究科, 准教授 (30398466)
SUZUKI ATSUSHI 横浜国立大学, 工学研究院, 准教授 (60467058)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生医療 / 骨 / 血管 / コラーゲン / 間葉系幹細胞 / 骨組織 |
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| Outline of Final Research Achievements |
Fabrication of three-dimensional vascularized bone graft is a major challenge in bone tissue engineering. A collagen gel solution containing bone marrow derived mesenchymal stem cells (MSCs) was dropped on a hydrophobic plate, which then spontaneously contracted and formed spherical shape during culture by contraction forces of MSCs. The surface of MSC-embedded microgel was covered with human umbilical vein endothelial cells. Transplantation of the microgels with endothelial cells into a bone-defeated model mouse revealed acceleration of bone regeneration with improved blood supply, compared to microgels without endothelial cells, clinically proven biomaterial (beta-TCP), and non-transplantation control. This approach may be a fundamental technique for engineering osteon-like tissues to bone regenerative medicine.
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Report
(3 results)
Research Products
(51 results)
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[Journal Article] The role of an octacalcium phosphate in the reformation of infraspinatus tendon insertion2015
Author(s)
Itoigawa Y, Suzuki O, Sano H, Anada T, Handa T, Hatta T, Kuwahara Y, Takahashi A, Ezoe Y, Kaneko K, Itoi E.
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Journal Title
J Shoulder Elbow Surg
Volume: in press
Related Report
Peer Reviewed
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