| Project/Area Number |
26630423
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Yokohama National University |
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Principal Investigator |
Fukuda Junji 横浜国立大学, 工学(系)研究科(研究院), 准教授 (80431675)
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| Co-Investigator(Kenkyū-buntansha) |
ANADA TAKAHISA 東北大学, 歯学研究科, 准教授 (30398466)
SUZUKI ATSUSHI 横浜国立大学, 工学研究院, 准教授 (60467058)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生医療 / 骨 / 血管 / コラーゲン / 間葉系幹細胞 / 骨組織 |
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| Outline of Final Research Achievements |
Fabrication of three-dimensional vascularized bone graft is a major challenge in bone tissue engineering. A collagen gel solution containing bone marrow derived mesenchymal stem cells (MSCs) was dropped on a hydrophobic plate, which then spontaneously contracted and formed spherical shape during culture by contraction forces of MSCs. The surface of MSC-embedded microgel was covered with human umbilical vein endothelial cells. Transplantation of the microgels with endothelial cells into a bone-defeated model mouse revealed acceleration of bone regeneration with improved blood supply, compared to microgels without endothelial cells, clinically proven biomaterial (beta-TCP), and non-transplantation control. This approach may be a fundamental technique for engineering osteon-like tissues to bone regenerative medicine.
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