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Development of a novel immunoassay system using NIR luminescent glass

Research Project

Project/Area Number 26630425
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionNagoya University

Principal Investigator

Nakano Hideo  名古屋大学, 生命農学研究科, 教授 (00237348)

Co-Investigator(Kenkyū-buntansha) 渕 真悟  青山学院大学, 理工学部, 准教授 (60432241)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords免疫測定 / 近赤外蛍光 / ルシフェラーゼ / 抗体 / 近赤外蛍光ガラス / 抗体酵素複合体 / 直中毒菌 / バイオ関連機器 / 近赤外光 / 発光タンパク質 / バイオセンサー
Outline of Final Research Achievements

We have developed a novel and rapid immunoassay system, named BINGO (Bioluminescent Interference Gathering Optical) assay, without any separation steps. It employs luciferase-antibody complex (LucAb). Initially, antibody-luciferase are uniformly dispersed in the solution. The luminescent excited by the luc was detected by photo-multiplier tube (PMT) for near-infrared light. Localization of antigen-antibody complex to the fluorescent glass increased the PMT signal. Then another system without the NIR-fluorescent glass was also tried. A PMT detector for visible light was equipped under the bottom, so the luminesce by Luc was directly detected. The principle was initially demonstrated in a model system, and it also successively detected E. coli O157 (toxin-defective) in a 30 min.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] Development of a novel immunosorbent homogeneous assay system using luciferase-antibody complex and dye for luminescence absorption.2016

    • Author(s)
      Mori, Teruyo Ojima-Kato, Shingo Fuchi, Takaaki Kojima, Hideo Nakano.
    • Organizer
      251st American Chemical Society National Meeting & Exposition
    • Place of Presentation
      San Diego
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 近赤外蛍光ガラスを用いた新規免疫学的検出手法の開発。2015

    • Author(s)
      森 昭博, 加藤 晃代, 渕 慎吾, 兒島 孝明, 中野 秀雄.
    • Organizer
      日本生物工学会平成25年度解体
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 免疫測定用キット及び免疫測定方法2015

    • Inventor(s)
      中野、森、加藤、渕
    • Industrial Property Rights Holder
      中野、森、加藤、渕
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-186581
    • Filing Date
      2015-09-24
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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