Development of protein transfection methods using stimuli responsive gels
| Project/Area Number |
26630431
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質導入 / 再生医療 / 界面活性剤 / 油水界面 / ナノ集合体 |
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| Outline of Final Research Achievements |
The cells for cell therapy have been generally prepared by gene transfection and protein expression. However, the gene transfection is difficult to obtain the stable protein expression and high safety. In this study, a Solid-in-Oil (S/O) suspension containing surfactant-coated proteins in oil phase was employed as the protein transfection tool. Since the coated proteins form a lipophilic nanoparticle, they have high compatibility with the cell membrane. Therefore,
the S/O suspension induced the acceleration of protein transfection.
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Report
(3 results)
Research Products
(4 results)
