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Development of protein transfection methods using stimuli responsive gels

Research Project

Project/Area Number 26630431
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionYamaguchi University

Principal Investigator

Toorisaka Eiichi  山口大学, 理工学研究科, 准教授 (40363543)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsタンパク質導入 / 再生医療 / 界面活性剤 / 油水界面 / ナノ集合体
Outline of Final Research Achievements

The cells for cell therapy have been generally prepared by gene transfection and protein expression. However, the gene transfection is difficult to obtain the stable protein expression and high safety. In this study, a Solid-in-Oil (S/O) suspension containing surfactant-coated proteins in oil phase was employed as the protein transfection tool. Since the coated proteins form a lipophilic nanoparticle, they have high compatibility with the cell membrane. Therefore,
the S/O suspension induced the acceleration of protein transfection.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Presentation (4 results)

  • [Presentation] 膜融合ナノ粒子を用いた動物細胞へのタンパク質導入2015

    • Author(s)
      萩原克樹,通阪栄一
    • Organizer
      化学工学会中国支部・関西支部合同支部大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 液/液界面を利用した細胞培養とタンパク質導入の検討2015

    • Author(s)
      萩原克樹,通阪栄一
    • Organizer
      日本膜学会第37年会
    • Place of Presentation
      早稲田大学(東京)
    • Year and Date
      2015-05-14 – 2015-05-15
    • Related Report
      2014 Research-status Report
  • [Presentation] 液/液界面を利用した細胞培養とタンパク質導入の検討2015

    • Author(s)
      萩原克樹,通阪栄一
    • Organizer
      日本膜学会第37年会
    • Place of Presentation
      早稲田大学(東京都新宿区)
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Protein transfection into mammalian cells with membrane fusion nanoparticles2014

    • Author(s)
      Katsuki Hagiwara, Eiichi Toorisaka
    • Organizer
      The 2nd International Symposium on Technologies for a Safe and Environmental Friendly Society
    • Place of Presentation
      Yamaguchi University (宇部市)
    • Year and Date
      2014-11-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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