• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of DDS system based on the intracellular enviroment responsive aptamers

Research Project

Project/Area Number 26630432
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionThe University of Tokushima

Principal Investigator

MINAKAWA Noriaki  徳島大学, 大学院医歯薬学研究部, 教授 (40209820)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Kazuhiro  徳島大学, 大学院医歯薬学研究部, 助教 (00644999)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsアプタマー / 薬剤放出システム / ヌクレオシド / 細胞環境応答 / 4'-セレノヌクレオチド
Outline of Final Research Achievements

In this work, we aimed to develop a novel DDS system based on the intracellular enviroment responsive aptamers, called PARCEL(Practical Aptamer Responding to CELlular Environment. In order to complete this project, we prepared 2'-deoxy-4'-selenothymidine 5'-triphosphate (SeTTP). Using the resulting SeTTP, PCR was investigated. As a result, PCR went well when KOD dash DNA polymerase was used in the presence of DMSO. Accordingly, we confirmed the conditions to obtain the desired PARCEL.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Synthesis of DNA fragments containing 2’-deoxy-4’-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4’-position in replication2015

    • Author(s)
      Noriko Tarashima, Tatsuya Sumitomo, Hidenori Ando, Kazuhiro Furukawa, Tatsuhiro Ishida and Noriaki Minakawa*
    • Journal Title

      Org.Biomol.Chem

      Volume: 13 Issue: 25 Pages: 6949-6952

    • DOI

      10.1039/c5ob00941c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Synthesis and properties of a novel 1,2-dithianenucleoside2014

    • Author(s)
      Tadashi Miyazawa, Noriko Tarashima, Kazuhiro Furukawa, Noriaki Minakawa
    • Organizer
      第41回国際核酸化学シンポジウム
    • Place of Presentation
      北九州国際会議場(福岡県北九州市)
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi