| Project/Area Number |
26630434
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | アカテガニ / セルラーゼ / リグニン / 腸内細菌 / グアヤコール / ヨコエビ / バイオマス / セルロース / Shewanella / Bacillus licheniformis |
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| Outline of Final Research Achievements |
Land crab, Chiromantes haematocheir mainly consume plant material and its detritus. They are expected to have cellulose and lignin degrading activities to digest plant materials. Isolation of biomass degrading bacteria was performed from gut of C. haematocheir. Fifteen kinds of bacteria were isolated under aerobic condition and 14 strains showed clear cellulase activities. These cellulase activities were detected in culture supernatants and were able to degrade carboxymethyl cellulose (CMC) to mono sugar units. Among 14 cellulase-producing bacteria, 2 strains (Bacillus licheniformis LB1 and Shewanella xiamensis LB5) seemed to show activities reacting lignin or lignin derivative compounds. B. licheniformis LB1 showed clear halo-forming activity on lignin containing plate and S. xiamensis LB5 exhibited high activity to react with guaiacol which is low molecular lignin derivative. Biomass-degrading bacteria might contribute to the ability of the land crab to digest biomass in gut.
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