| Project/Area Number |
26630435
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Hara Masayuki 大阪府立大学, 理学(系)研究科(研究院), 教授 (50344172)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Hideki 大阪府立大学, 大学院理学系研究科, 准教授 (30450894)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | mesenchymal stem cell / bone / photosensitizer / hematorporphyrin / rhodamine / reative oxygen species / hematoporphyrin / reactive oxygen species |
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| Outline of Final Research Achievements |
We studied about effect of reactive oxygen species (ROS) evolved by visible light-activated photosensitizer (PS) on the cultured mesenchymal stem cells (MSCs) derived from rat bone marrow. White light from light emitting diode (LED) was used for activation of PS in the experiments. MSCs proliferated well in the growth medium and differentiated into osteoblasts in the osteogenic culture medium. Gene expression levels were measured by quantitative RT-PCR method after the MSCs cultured in both media were exposed to moderate level of ROS. Genes concerning osteogenic, condrogenic and adipogenic differentiation in MSCs were up-regulated. Then we considered that moderate oxidative stress not strong enough to kill the cultured MSCs promoted the differentiation of MSCs.
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