Construction of in vivo high-throughput screening system for bioactive metabolites
| Project/Area Number |
26630436
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ラマン分光 / 生理活性物質 / 非侵襲 / マイクロ流体デバイス / スクリーニング |
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| Outline of Final Research Achievements |
In this research, we aimed to develop a novel technique for screening of bioactive compound producers from environmental bacteria without bacterial cultivation or extraction and purification of the compounds. In order to detect bioactive compounds non-invasively, we firstly tried to obtain Raman spectral library of biomolecules. Based on this data library, we have succeeded in in situ detection of the Raman bands derived to antibiotics with the aid of resonance Raman effect and multivariate analysis technique. In addition, to achieve the high-throughput screening of bioactive compounds from environmental bacteria, we have developed the microfluidic bacterial culture and Raman spectral measurement systems and evaluated the performance of them.
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Report
(3 results)
Research Products
(7 results)
