| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Increases in intracellular chloride ion concentrations of neurons following inhibitory postsynaptic potentials (IPSPs) have not been visualized to date. In this study, aiming at detecting IPSPs, we developed a genetically encoded chloride probe with high responsivity. The probe showed a fairly large fluorescence change in response to chloride in vitro. Then we expressed the probe in neuronal cells and assessed its functionalities, focusing on responses to inhibitory inputs.
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