Development of a novel chloride probe aiming at detecting inhibitory synaptic inputs
Project/Area Number |
26640002
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Saitama University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 神経科学 / 神経活動 / 抑制性シナプス / クロライドプローブ / 蛍光イメージング |
Outline of Final Research Achievements |
Increases in intracellular chloride ion concentrations of neurons following inhibitory postsynaptic potentials (IPSPs) have not been visualized to date. In this study, aiming at detecting IPSPs, we developed a genetically encoded chloride probe with high responsivity. The probe showed a fairly large fluorescence change in response to chloride in vitro. Then we expressed the probe in neuronal cells and assessed its functionalities, focusing on responses to inhibitory inputs.
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Report
(3 results)
Research Products
(51 results)
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[Journal Article] A top-down cortical circuit for accurate sensory perception2015
Author(s)
Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
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Journal Title
Neuron
Volume: 86(5)
Issue: 5
Pages: 1304-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
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Journal Title
Nat Methods
Volume: 12
Issue: 1
Pages: 64-70
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Water-soluble jack-knife prawn extract inhibits 5-hydroxytryptamine-induced vasoconstriction and platelet aggregation in humans.2015
Author(s)
Gamoh S, Kanai T, Tanaka-Totoribe N, Ohkura M, Kuwabara M, Nakamura E, Yokota A, Yamasaki T, Watanabe A, Hayashi M, Fujimoto S, *Yamamoto R
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Journal Title
Food Funct
Volume: 6
Issue: 2
Pages: 444-449
DOI
Related Report
Peer Reviewed
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[Presentation] 意識下活動動物からの特定神経活動記録法の開発.2016
Author(s)
山下哲, 犬束歩, Srikanta Chowdhury, 中井淳一, 大倉正道, 田口徹, 桑木共之, 山中章弘
Organizer
第93回日本生理学会大会
Place of Presentation
札幌コンベンションセンター (北海道札幌市)
Year and Date
2016-03-22
Related Report
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[Presentation] Rational design of ultrafast, high-affinity calcium indicators for monitoring neuronal activity.2015
Author(s)
Masatoshi Inoue, Atsuya Takeuchi, Shin-ichiro Horigane, Hajime Fujii, Satoshi Kamijo, Sayaka Takemoto-Kimura, Masamichi Ohkura, Keiko Gengyo-Ando, Masanobu Kano, Junichi Nakai, Kazuo Kitamura, and Haruhiko Bito
Organizer
Neuroscience 2015
Place of Presentation
McCormick Place (Chicago, USA)
Year and Date
2015-10-17
Related Report
Int'l Joint Research
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[Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP2.2015
Author(s)
Masatoshi Inoue, Atsuya Takeuchi, Shin-ichiro Horigane, Hajime Fujii, Satoshi Kamijo, Sayaka Takemoto-Kimura, Masamichi Ohkura, Keiko Gengyo-Ando, Masanobu Kano, Junichi Nakai, Kazuo Kitamura, Haruhiko Bito
Organizer
第58回日本神経化学大会
Place of Presentation
大宮ソニックシティ (埼玉県さいたま市)
Year and Date
2015-09-11
Related Report
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[Presentation] Cortical top-down inputs for precise sensory perception in mice.2015
Author(s)
Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
Organizer
第38回日本神経科学大会シンポジウム
Place of Presentation
神戸国際会議場 (兵庫県神戸市)
Year and Date
2015-07-28
Related Report
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[Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP2.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
Organizer
第38回日本神経科学大会
Place of Presentation
神戸国際会議場 (兵庫県神戸市)
Year and Date
2015-07-28
Related Report
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[Presentation] 高感度、超高速赤色カルシウムインディケーターの合理的設計による開発.2015
Author(s)
井上昌俊, 竹内敦也, 堀金慎一郎, 大倉正道, 安藤恵子, 藤井哉, 上條諭志, 竹本-木村さやか, 狩野方伸, 中井淳一, 喜多村和郎, 尾藤晴彦
Organizer
第92回日本生理学会大会
Place of Presentation
神戸国際会議場(兵庫県神戸市)
Year and Date
2015-03-21 – 2015-03-23
Related Report
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[Presentation] マウス皮質において正確な知覚に必要なトップダウン制御のメカニズム.2015
Author(s)
真仁田聡, 鈴木崇之, 本間千尋, 松元崇, 小田川摩耶, 山田一之, 太田桂輔, 松原智恵, 犬束歩, 佐藤正晃, 大倉正道, 山中章弘, 柳川右千夫, 中井淳一, 林康紀, Matthew E. Larkum, 村山正宜
Organizer
第92回日本生理学会大会
Place of Presentation
神戸国際会議場(兵庫県神戸市)
Year and Date
2015-03-21 – 2015-03-23
Related Report
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[Presentation] 海馬CA1における価値表現の選択的保持.2015
Author(s)
水田恒太郎, 佐藤正晃, 関根友紀子, 河野真子, イスラムタンビル, 大倉正道, 中井淳一, 林康紀
Organizer
第92回日本生理学会大会
Place of Presentation
神戸国際会議場(兵庫県神戸市)
Year and Date
2015-03-21 – 2015-03-23
Related Report
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[Presentation] 高感度、超高速赤色カルシウムインディケーターの合理的設計による開発.2015
Author(s)
井上昌俊, 竹内敦也, 堀金慎一郎, 大倉正道, 安藤恵子, 藤井哉, 上條諭志, 竹本-木村さやか, 狩野方伸, 中井淳一, 喜多村和郎, 尾藤晴彦
Organizer
第88回日本薬理学会年会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2015-03-18 – 2015-03-20
Related Report
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[Presentation] 大脳皮質におけるトップダウン回路の構造と機能.2014
Author(s)
真仁田聡, 鈴木崇之, 本間千尋, 松元崇, 小田川摩耶, 山田一之, 太田桂輔, 松原智恵, 犬束歩, 佐藤正晃, 大倉正道, 山中章弘, 柳川右千夫, 中井淳一, 林康紀, Matthew E. Larkum, 村山正宜
Organizer
第37回日本神経科学大会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2014-09-11 – 2014-09-13
Related Report
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[Presentation] Ca2+イメージング.2014
Author(s)
大倉正道
Organizer
第39回組織細胞化学講習会
Place of Presentation
ピアザ淡海(滋賀県大津市)
Year and Date
2014-08-06 – 2014-08-08
Related Report
Invited
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[Presentation] Cerebral blood flow modulation by basal forebrain or whisker stimulation can occur independently of large cytosolic Ca2+ signaling in astrocytes.2014
Author(s)
Oe Y, Ozawa K, Takata N, Nagai T, Konno A, Ohkura M, Hirai H, Nakai J, Mikoshiba K, Hirase H
Organizer
9th FENS Forum of Neuroscience
Place of Presentation
MiCo (Milan, Italy)
Year and Date
2014-07-05 – 2014-07-09
Related Report
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