| Project/Area Number |
26640008
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | University of Toyama |
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Principal Investigator |
OHKAWA Noriaki 富山大学, 大学院医学薬学研究部(医学), 講師 (80416651)
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| Co-Investigator(Renkei-kenkyūsha) |
INOKUCHI Kaoru 富山大学, 大学院医学薬学研究部(医学), 教授 (20318827)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | Ca2+イメージング / 学習 / 記憶 / 記憶痕跡細胞 / 神経活動 / 遺伝子発現 / レンチウイルス / 記憶痕跡 |
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| Outline of Final Research Achievements |
To identify rules for expression of engram, we proposed to establish a new technique to observe activity pattern of engram cells from pre- to post-learning phase.
We installed a GRIN lens on hippocampal CA1, and a miniature microscope detected fluorescence signals in the CA1 through the GRIN lens. Then we applied the microscopy on transgenic mice in which hippocampal neurons express G-CaMP and engram cells express a fluorescence protein, KikGR. The G-CaMP is a calcium indicator and shows fluorescence when neural activity induces calcium influx into the CA1 neurons. We have succeeded to observe both of engram cells expressing the KikGR and activity-mediated G-CaMP fluorescence of ~400 neurons from freely moving mice with the miniature microscope.
The new technique will allow to investigate comparisons of activity pattern between engram cells and others during memory processing.
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