Application of the photo-activatable transcriptional factor to neural circuit study

• Project/Area Number: 26640011
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: IMAYOSHI Itaru 京都大学, 白眉センター, 准教授 (60543296)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 光応答性転写因子 / ニューロン / 神経幹細胞

Outline of Final Research Achievements

We conducted fundamental tool developments of photo manipulation of the gene expression. We optimized photo-activatable transcriptional factors for neural circuit studies. We generated a series of lenti virus vectors that can express GAVPO and UAS-downstream genes under the control of blue light.