| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
By conducting DNA methylation analysis from the embryo to the postnatal brain, establishment of the iPS cell line from the embryonic lethal mouse and generation of the chimeric mouse, and gene expression analysis and morphological analysis at the single neuron level, we revealed that differential DNA methylation dependent on DNA methylation enzyme Dnmt3b regulates the stochastic expression of the clustered protocadherin genes in Purkinje cells and that it is involved in pattern formation of dendrites. In addition, as a collaborative research, we found that the epigenetic regulation at the developmental stage is also involved in the formation of reciprocal synaptic connections dependent on the cell lineage of the cerebral cortex by performing double whole cell recording in the brain of chimeric mice.
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