| Project/Area Number |
26640013
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | 地方独立行政法人佐賀県医療センター好生館(ライフサイエンス研究所) |
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Principal Investigator |
KANKI Hiroaki 地方独立行政法人佐賀県医療センター好生館(ライフサイエンス研究所), ライフサイエンス研究所, 研究員 (80321751)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 神経幹細胞 / ニッチ / 幹細胞 / 神経新生 / ニッシェ |
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| Outline of Final Research Achievements |
As a pilot system for precise identification of neural (or other cell types of) stem cell niches in adult mammals, I tried in this study to develop a visualization system for “neural stem cell (NSC)-surrounding cells,” utilizing fluorescent proteins. Briefly, a fluorescent peptide is to be secreted from NSCs and then immediately taken up by the surrounding cells, which are a counterpart for NSC niche-constituting cells. Through a set of evaluation processes, I confirmed that NSC-surrounding cells were indeed fluorescently-labeled, as planned. The intensity of the fluorescence, however was much lower than expected, suggesting that the system needs additional modifications.
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