Multiple cell membrane potential measurement by a random-scan two-photon microscope
| Project/Area Number |
26640016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 膜電位測定 / 電位感受性色素 / 2光子励起顕微鏡 / 脳スライス / 多点膜電位計測 / シナプス結合 |
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| Outline of Final Research Achievements |
This research project aimed to measure membrane potential changes from multiple neurons simultaneously by means of an in-house developed random-scan two -photon microscope. For this purpose, I developed algorithms for extraction of spikes from time course changes of fluorescence intensity and for temporal correlation of spike events of pairs of neurons, which were implemented to analysis software. With these analysis methods enabled successful extraction of neuron spikes and determination of synaptic connection and even the direction of synaptic connections of every neuron pair. I was also successful in development of a staining method of neurons in brain slice preparations. With these developments of methods I succeeded in measuring action potentials optically from multiple neurons, e.g., cerebellar granule cells and neocortical neurons in brain slice preparations.
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Report
(4 results)
Research Products
(1 results)
