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Multiple cell membrane potential measurement by a random-scan two-photon microscope

Research Project

Project/Area Number 26640016
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionWaseda University

Principal Investigator

Inoue Takafumi  早稲田大学, 理工学術院, 教授 (10262081)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords膜電位測定 / 電位感受性色素 / 2光子励起顕微鏡 / 脳スライス / 多点膜電位計測 / シナプス結合
Outline of Final Research Achievements

This research project aimed to measure membrane potential changes from multiple neurons simultaneously by means of an in-house developed random-scan two -photon microscope. For this purpose, I developed algorithms for extraction of spikes from time course changes of fluorescence intensity and for temporal correlation of spike events of pairs of neurons, which were implemented to analysis software. With these analysis methods enabled successful extraction of neuron spikes and determination of synaptic connection and even the direction of synaptic connections of every neuron pair. I was also successful in development of a staining method of neurons in brain slice preparations. With these developments of methods I succeeded in measuring action potentials optically from multiple neurons, e.g., cerebellar granule cells and neocortical neurons in brain slice preparations.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Optical detection of neuron connectivity by random access two-photon microscopy2016

    • Author(s)
      Nasrin Shafeghat, Morteza Heidarinejad, Noboru Murata, Hideki Nakamura, Takafumi Inoue
    • Journal Title

      Journal of Neuroscience Methods

      Volume: 263 Pages: 48-56

    • DOI

      10.1016/j.jneumeth.2016.01.023

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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