Discrimination of sensory signaling in pheromone and temperature-sensing neuron

• Project/Area Number: 26640018
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Konan University
• Principal Investigator: Kuhara Atsuhsi 甲南大学, 理工学部, 准教授 (00402412)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: C. elegans / 区別識別 / 線虫 / 3量体Gタンパク質 / カルシウムイメージング / 神経情報の識別 / 神経情報処理

Outline of Final Research Achievements

How multiple neural signaling is discriminate and crosstalk in the neuron is important question. Here we established experimental system for analyzing their processing of multiple neural signaling. We found that a sensoryneuron known as pheromone and light sensing-neuron acts as a thermosensoryneuron. In this multi stimuli common sensoryneuron, trimeric G protein, our culcium imaging revealed that guanylyl cyclase and phosphodiesterase regulates pheromone, light and temperature signaling. However, we found that GPA-1 encoding G protein alpha subunit and PDE-3 encoding phosphodiesterase control temperature signaling specifically. These results suggest that commonalities and differences in the molecular components concerned with pheromone, light and temperature signaling in a single sensory neuron.

Research Products

• [Int'l Joint Research] アラスカ大学/カリフォルニア大学(米国)
• [Journal Article] Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. (2016)
  • Author(s): Ujisawa, T., Ohta, A., Uda-Yagi, M., Kuhara A.
  • Journal Title: PLOS ONE Volume: 11(10) Issue: 10 Pages: 1-20
  • DOI: 10.1371/journal.pone.0165518
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sperm affects head sensory neuron in temperature tolerance of Caenorhabditis elegans (2016)
  • Author(s): Sonoda S., Ohta, A., Maruo, A., Ujisawa T., Kuhara, A.
  • Journal Title: Cell Reports Volume: 16(1) Issue: 1 Pages: 56-65
  • DOI: 10.1016/j.celrep.2016.05.078
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Natural variations of cold tolerance and temperature acclimation in Caenorhabditis elegans (2016)
  • Author(s): Okahata M., Ohta, A., Mizutani, H., Minakuchi, Y., Toyoda, A., Kuhara, A.
  • Journal Title: Journal of Comparative Physiology B. Volume: 186(8) Issue: 8 Pages: 985-998
  • DOI: 10.1007/s00360-016-1011-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Caenorhabditis elegans homolog of Prox1/Prospero is expressed in the glia and is required for sensory behavior and cold tolerance. (2016)
  • Author(s): Kage-Nakadai E., Ohta, A., Ujisawa T., Sun S., Nishikawa Y., Kuhara A., Mitani S.
  • Journal Title: Genes to Cells Volume: 21(9) Issue: 9 Pages: 936-948
  • DOI: 10.1111/gtc.12394
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans. (2016)
  • Author(s): Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
  • Journal Title: J Neurosci. Volume: 36 Issue: 9 Pages: 2571-2581
  • DOI: 10.1523/jneurosci.2837-15.2016
  • NAID: 120006360790
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 線虫 Caenorhabditis elegans の温度適応を制御する神経と腸を介した情報処理 (2015)
  • Author(s): 久原 篤, 宇治澤 知代, 太田 茜
  • Journal Title: 比較生理生化学 Volume: 32 Pages: 67-75
  • NAID: 130005078135
  • Peer Reviewed
• [Journal Article] Light and pheromone-sensing neuron regulates cold habituation through insulin signaling in C. elegans (2014)
  • Author(s): Ohta, A., Ujisawa T., Sonoda S., Kuhara, A.
  • Journal Title: Nature commun. Volume: 5:4412 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms5412
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term calcium imaging of ASJ sensoryneuron controlling cold tolerance in C. elegans (2014)
  • Author(s): Ujisawa T., Ohta, A., Kuhara, A.
  • Journal Title: Protocol Exchange Volume: 0 Pages: 1-6
  • DOI: 10.1038/protex.2014.034
  • Open Access / Acknowledgement Compliant
• [Presentation] 温度応答を制御する分子機構 (2017)
  • Author(s): 宇治澤 知代、太田茜、久原篤
  • Organizer: 温度生物学 第2回若手の会
  • Place of Presentation: ホテル東海園(蒲郡市、愛知)
• [Presentation] 線虫から学ぶ温度応答の制御機構 (2016)
  • Author(s): 久原篤, 太田茜
  • Organizer: 第1回Biothermology Workshop - 生命システムの熱科学 -
  • Place of Presentation: 岡崎コンファレンスセンター(岡崎、愛知)
  • Year and Date: 2016-12-10
  • Invited
• [Presentation] Molecular logic for temperature signaling in cold tolerance of C. elegans. (2016)
  • Author(s): Tomoyo UJISAWA, Misato UDA，Akane OHTA，Katsushi ARISAKA，Atsushi KUHARA
  • Organizer: 生物物理学会
  • Place of Presentation: 筑波国際会議場(つくば市、茨城)
  • Year and Date: 2016-11-25
• [Presentation] Molecular neural circuit mechanisms underlying temperature acclimation speed in C. elegans (2016)
  • Author(s): Akane Ohta, Makoto Ioroi, Satoko Fujii, Atsushi Kuhara
  • Organizer: ICZ22 ZSJ87 jointmeeting 2016
  • Place of Presentation: 沖縄コンベンションセンター(宜野湾、Okinawa)
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] Molecular physiological mechanism of cold tolerance (2016)
  • Author(s): Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara
  • Organizer: C. elegans Neuronal Development, Synaptic Function & Behavior meeting
  • Place of Presentation: C. elegans Neuronal Development, Synaptic Function & Behavior meeting
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Molecular neuro-mechanism underlying temperature tolerance in C. elegans (2016)
  • Author(s): Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara
  • Organizer: 神経科学学会
  • Place of Presentation: パシフィコ横浜(横浜、神奈川)
  • Year and Date: 2016-07-20
• [Presentation] 線虫から探る温度応答と記憶のエッセンス (2015)
  • Author(s): 久原篤、宇治澤知代、太田茜
  • Organizer: 動物学会近畿支部会 秋期講習会
  • Place of Presentation: 甲南大学、神戸
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] 線虫の温度適応に関わるGPCR型の温度受容体のスクリーニング (2015)
  • Author(s): 大西 康平、三浦 徹、宇治澤 知代、宇多 美里、太田 茜、久原 篤
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-12-01
• [Presentation] 温度適応における温度受容ニューロンと下流組織の分子制御機構 (2015)
  • Author(s): 宇治澤 知代, 宇多美里、井伊辰也、井伊美紀、太田 茜, 久原 篤
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-12-01
• [Presentation] 線虫から学ぶ温度に対する生体調節 (2015)
  • Author(s): 久原 篤、宇治澤知代、太田 茜
  • Organizer: 動物学会 ホメオスタシスバイオロジーシンポジウム
  • Place of Presentation: 朱鷺メッセ、新潟
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] Temperature experience-dependent cold acclimation in nematode C. elegans (2015)
  • Author(s): Tomoyo Ujisawa, Kohei Ohnishi, Tohru Miura, Akane Ohta, Atsushi Kuhara
  • Organizer: 生物物理学会
  • Place of Presentation: 金沢大学、金沢
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Temperature experience-dependent cold habituation in C. elegans (2015)
  • Author(s): Atsushi Kuhara, Tomoyo Ujisawa, Tohru Miura, Misato Uda, Akane Ohta
  • Organizer: 神経科学学会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-07-28
• [Presentation] フェロモンや光感知ニューロンにおける温度感知が個体の温度適応を支配する (2014)
  • Author(s): 宇治澤知代、太田茜、園田悟、久原篤
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-27
• [Presentation] light and pheromone sensoryneuron regulates temperature habituation in C. elegans (2014)
  • Author(s): Atsushi Kuhara, Tomoyo Ujisawa, Satoru Sonoda, Misaki Okahata, Akane Ohta
  • Organizer: ISMNTOP2014(The 12th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (YR Umami Forum 2014))
  • Place of Presentation: 九州大学
  • Year and Date: 2014-11-02 – 2014-11-03
  • Invited
• [Presentation] 線虫C. elegansにおける温度適応を制御する神経システム (2014)
  • Author(s): 宇治澤 知代、園田 悟、太田茜、久原 篤
  • Organizer: 遺伝学会
  • Place of Presentation: 長浜バイオ大学
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] Temperature habituation is regulated by light and pheromone sensoryneuron in C. elegans (2014)
  • Author(s): Tomoyo Ujisawa, Akane Ohta, Atsushi Kuhara
  • Organizer: 神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-15
• [Presentation] Systematic regulation for temperature experience-dependent cold habituation (2014)
  • Author(s): Akane Ohta, Tomoyo Ujisawa, Misato Uda, Tomohiro Ishiwari, Makoto Ioroi, Natsune Takagaki, Mai Kimura, Satoru Sonoda, Atsushi Kuhara
  • Organizer: C.elegans Development, Cell Biology & Gene Expression Meeting / 6th Asia-Pacific C.elegans Meeting
  • Place of Presentation: Nara
  • Year and Date: 2014-07-15 – 2014-07-22
• [Presentation] 線虫C. elegansの温度応答-適応における3量体Gタンパク質を介した情報伝達 (2014)
  • Author(s): 宇多美里、宇治澤知代、石割友博、木村 真衣、太田茜、久原篤
  • Organizer: 動物学会近畿支部会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2014-05-10
• [Book] 研究者が教える動物実験 第3巻 行動 (p32-35担当) (2015)
  • Author(s): 太田茜、園田悟、久原 篤
  • Total Pages: 222
  • Publisher: 共立出版
• [Book] 研究者が教える動物実験 第3巻 行動 (p36-39担当) (2015)
  • Author(s): 太田茜、園田悟、久原 篤
  • Total Pages: 222
  • Publisher: 共立出版
• [Book] 研究者が教える動物実験 第1巻 感覚 (p42-45担当) (2015)
  • Author(s): 太田茜、園田悟、久原 篤
  • Total Pages: 199
  • Publisher: 共立出版
• [Remarks] 久原研究室ホームページ
  • URL: http://kuharan.com/index.html
• [Remarks] 所属大学研究者紹介
  • URL: http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html
• [Remarks] 甲南大学研究者紹介
  • URL: http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html