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Discrimination of sensory signaling in pheromone and temperature-sensing neuron

Research Project

Project/Area Number 26640018
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionKonan University

Principal Investigator

Kuhara Atsuhsi  甲南大学, 理工学部, 准教授 (00402412)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsC. elegans / 区別識別 / 線虫 / 3量体Gタンパク質 / カルシウムイメージング / 神経情報の識別 / 神経情報処理
Outline of Final Research Achievements

How multiple neural signaling is discriminate and crosstalk in the neuron is important question. Here we established experimental system for analyzing their processing of multiple neural signaling. We found that a sensoryneuron known as pheromone and light sensing-neuron acts as a thermosensoryneuron. In this multi stimuli common sensoryneuron, trimeric G protein, our culcium imaging revealed that guanylyl cyclase and phosphodiesterase regulates pheromone, light and temperature signaling. However, we found that GPA-1 encoding G protein alpha subunit and PDE-3 encoding phosphodiesterase control temperature signaling specifically. These results suggest that commonalities and differences in the molecular components concerned with pheromone, light and temperature signaling in a single sensory neuron.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (33 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 6 results) Presentation (18 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (3 results) Remarks (3 results)

  • [Int'l Joint Research] アラスカ大学/カリフォルニア大学(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.2016

    • Author(s)
      Ujisawa, T., Ohta, A., Uda-Yagi, M., Kuhara A.
    • Journal Title

      PLOS ONE

      Volume: 11(10) Issue: 10 Pages: 1-20

    • DOI

      10.1371/journal.pone.0165518

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sperm affects head sensory neuron in temperature tolerance of Caenorhabditis elegans2016

    • Author(s)
      Sonoda S., Ohta, A., Maruo, A., Ujisawa T., Kuhara, A.
    • Journal Title

      Cell Reports

      Volume: 16(1) Issue: 1 Pages: 56-65

    • DOI

      10.1016/j.celrep.2016.05.078

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Natural variations of cold tolerance and temperature acclimation in Caenorhabditis elegans2016

    • Author(s)
      Okahata M., Ohta, A., Mizutani, H., Minakuchi, Y., Toyoda, A., Kuhara, A.
    • Journal Title

      Journal of Comparative Physiology B.

      Volume: 186(8) Issue: 8 Pages: 985-998

    • DOI

      10.1007/s00360-016-1011-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Caenorhabditis elegans homolog of Prox1/Prospero is expressed in the glia and is required for sensory behavior and cold tolerance.2016

    • Author(s)
      Kage-Nakadai E., Ohta, A., Ujisawa T., Sun S., Nishikawa Y., Kuhara A., Mitani S.
    • Journal Title

      Genes to Cells

      Volume: 21(9) Issue: 9 Pages: 936-948

    • DOI

      10.1111/gtc.12394

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans.2016

    • Author(s)
      Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
    • Journal Title

      J Neurosci.

      Volume: 36 Issue: 9 Pages: 2571-2581

    • DOI

      10.1523/jneurosci.2837-15.2016

    • NAID

      120006360790

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 線虫 Caenorhabditis elegans の温度適応を制御する神経と腸を介した情報処理2015

    • Author(s)
      久原 篤, 宇治澤 知代, 太田 茜
    • Journal Title

      比較生理生化学

      Volume: 32 Pages: 67-75

    • NAID

      130005078135

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Light and pheromone-sensing neuron regulates cold habituation through insulin signaling in C. elegans2014

    • Author(s)
      Ohta, A., Ujisawa T., Sonoda S., Kuhara, A.
    • Journal Title

      Nature commun.

      Volume: 5:4412 Issue: 1 Pages: 1-12

    • DOI

      10.1038/ncomms5412

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Long-term calcium imaging of ASJ sensoryneuron controlling cold tolerance in C. elegans2014

    • Author(s)
      Ujisawa T., Ohta, A., Kuhara, A.
    • Journal Title

      Protocol Exchange

      Volume: 0 Pages: 1-6

    • DOI

      10.1038/protex.2014.034

    • Related Report
      2014 Research-status Report
    • Open Access / Acknowledgement Compliant
  • [Presentation] 温度応答を制御する分子機構2017

    • Author(s)
      宇治澤 知代、太田茜、久原篤
    • Organizer
      温度生物学 第2回若手の会
    • Place of Presentation
      ホテル東海園(蒲郡市、愛知)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 線虫から学ぶ温度応答の制御機構2016

    • Author(s)
      久原篤, 太田茜
    • Organizer
      第1回Biothermology Workshop - 生命システムの熱科学 -
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎、愛知)
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Molecular logic for temperature signaling in cold tolerance of C. elegans.2016

    • Author(s)
      Tomoyo UJISAWA, Misato UDA,Akane OHTA,Katsushi ARISAKA,Atsushi KUHARA
    • Organizer
      生物物理学会
    • Place of Presentation
      筑波国際会議場(つくば市、茨城)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular neural circuit mechanisms underlying temperature acclimation speed in C. elegans2016

    • Author(s)
      Akane Ohta, Makoto Ioroi, Satoko Fujii, Atsushi Kuhara
    • Organizer
      ICZ22 ZSJ87 jointmeeting 2016
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾、Okinawa)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular physiological mechanism of cold tolerance2016

    • Author(s)
      Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara
    • Organizer
      C. elegans Neuronal Development, Synaptic Function & Behavior meeting
    • Place of Presentation
      C. elegans Neuronal Development, Synaptic Function & Behavior meeting
    • Year and Date
      2016-07-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular neuro-mechanism underlying temperature tolerance in C. elegans2016

    • Author(s)
      Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara
    • Organizer
      神経科学学会
    • Place of Presentation
      パシフィコ横浜(横浜、神奈川)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 線虫から探る温度応答と記憶のエッセンス2015

    • Author(s)
      久原篤、宇治澤知代、太田茜
    • Organizer
      動物学会近畿支部会 秋期講習会
    • Place of Presentation
      甲南大学、神戸
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 線虫の温度適応に関わるGPCR型の温度受容体のスクリーニング2015

    • Author(s)
      大西 康平、三浦 徹、宇治澤 知代、宇多 美里、太田 茜、久原 篤
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸国際会議場、神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 温度適応における温度受容ニューロンと下流組織の分子制御機構2015

    • Author(s)
      宇治澤 知代, 宇多美里、井伊辰也、井伊美紀、太田 茜, 久原 篤
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸国際会議場、神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 線虫から学ぶ温度に対する生体調節2015

    • Author(s)
      久原 篤、宇治澤知代、太田 茜
    • Organizer
      動物学会 ホメオスタシスバイオロジーシンポジウム
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Temperature experience-dependent cold acclimation in nematode C. elegans2015

    • Author(s)
      Tomoyo Ujisawa, Kohei Ohnishi, Tohru Miura, Akane Ohta, Atsushi Kuhara
    • Organizer
      生物物理学会
    • Place of Presentation
      金沢大学、金沢
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Temperature experience-dependent cold habituation in C. elegans2015

    • Author(s)
      Atsushi Kuhara, Tomoyo Ujisawa, Tohru Miura, Misato Uda, Akane Ohta
    • Organizer
      神経科学学会
    • Place of Presentation
      神戸国際会議場、神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] フェロモンや光感知ニューロンにおける温度感知が個体の温度適応を支配する2014

    • Author(s)
      宇治澤知代、太田茜、園田悟、久原篤
    • Organizer
      分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] light and pheromone sensoryneuron regulates temperature habituation in C. elegans2014

    • Author(s)
      Atsushi Kuhara, Tomoyo Ujisawa, Satoru Sonoda, Misaki Okahata, Akane Ohta
    • Organizer
      ISMNTOP2014(The 12th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (YR Umami Forum 2014))
    • Place of Presentation
      九州大学
    • Year and Date
      2014-11-02 – 2014-11-03
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 線虫C. elegansにおける温度適応を制御する神経システム2014

    • Author(s)
      宇治澤 知代、園田 悟、太田茜、久原 篤
    • Organizer
      遺伝学会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Temperature habituation is regulated by light and pheromone sensoryneuron in C. elegans2014

    • Author(s)
      Tomoyo Ujisawa, Akane Ohta, Atsushi Kuhara
    • Organizer
      神経科学学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-11 – 2014-09-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Systematic regulation for temperature experience-dependent cold habituation2014

    • Author(s)
      Akane Ohta, Tomoyo Ujisawa, Misato Uda, Tomohiro Ishiwari, Makoto Ioroi, Natsune Takagaki, Mai Kimura, Satoru Sonoda, Atsushi Kuhara
    • Organizer
      C.elegans Development, Cell Biology & Gene Expression Meeting / 6th Asia-Pacific C.elegans Meeting
    • Place of Presentation
      Nara
    • Year and Date
      2014-07-15 – 2014-07-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 線虫C. elegansの温度応答-適応における3量体Gタンパク質を介した情報伝達2014

    • Author(s)
      宇多美里、宇治澤知代、石割友博、木村 真衣、太田茜、久原篤
    • Organizer
      動物学会近畿支部会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Book] 研究者が教える動物実験 第3巻 行動 (p32-35担当)2015

    • Author(s)
      太田茜、園田悟、久原 篤
    • Total Pages
      222
    • Publisher
      共立出版
    • Related Report
      2015 Research-status Report
  • [Book] 研究者が教える動物実験 第3巻 行動 (p36-39担当)2015

    • Author(s)
      太田茜、園田悟、久原 篤
    • Total Pages
      222
    • Publisher
      共立出版
    • Related Report
      2015 Research-status Report
  • [Book] 研究者が教える動物実験 第1巻 感覚 (p42-45担当)2015

    • Author(s)
      太田茜、園田悟、久原 篤
    • Total Pages
      199
    • Publisher
      共立出版
    • Related Report
      2015 Research-status Report
  • [Remarks] 久原研究室ホームページ

    • URL

      http://kuharan.com/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report 2014 Research-status Report
  • [Remarks] 所属大学研究者紹介

    • URL

      http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
  • [Remarks] 甲南大学研究者紹介

    • URL

      http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-01-27  

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