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The mechanism of the cerebral cortical formation by genome replacement

Research Project

Project/Area Number 26640028
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

OKADO Haruo  公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, プロジェクトリーダー (60221842)

Co-Investigator(Kenkyū-buntansha) HIRAI Shinobu  公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (00625189)
HIRAI Sayaka  公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (80606434)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords脳形成 / RP58 / 大脳皮質 / OSVZ / ゲノム置換 / ヒトBac / 大傍皮質 / 転写抑制因子
Outline of Final Research Achievements

We generated the transgenic mice with human Bac clone containing RP58 gene ( 204 kbps). By mating them with RP58 hero mice, we generated the mice with human Bac of RP58 gene lacking mouse RP58. These mice show severe motor uncontrolled, suggesting that the function of cerebellum is impaired. Therefore, we tried to generate the mice lacking mouse RP58 only in cereberal cortex with RP58 flox mice and EmxCre mice. We generated the five strains of mice with RP58 negative, but human RP58 positive in the cereberal cortex. We are analyzing behavior of the mice, and plan to perform histological analysis.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2015 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Molecular Pathways Underlying Projection Neuron Production and Migration during Cerebral Cortical Development2015

    • Author(s)
      Ohtaka-Maruyama, C and Haruo Okado
    • Journal Title

      Frontiers in Neuroscience

      Volume: 9 Pages: 447-447

    • DOI

      10.3389/fnins.2015.00447

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development.2013

    • Author(s)
      Heng JI, Qu Z, Ohtaka-Maruyama C, Okado H, Kasai M, Castro D, Guillemot F, Tan SS.
    • Journal Title

      Cereb Cortex.

      Volume: - Issue: 3 Pages: 806-816

    • DOI

      10.1093/cercor/bht277

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 組織分化の複雑化にともなって発達したPOKファミリー分子群2015

    • Author(s)
      岡戸晴生
    • Organizer
      第38回日本分子生物学会(神戸)2015年、12月3日 ワークショップ
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大脳皮質の発生にはRP58による適切な転写抑制を必要とする2015

    • Author(s)
      平井 志伸,神嵜 誠司,岡戸 晴生
    • Organizer
      第38回日本分子生物学会(神戸)2015年、12月3日
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional Analysis of Mutated RP58/ZNF238 Observed in Patients with Intellectual Disability2015

    • Author(s)
      Kanzaki Seiji, Hirai-Sakamoto Shinobu, Hirai Sayaka, Shinmbo Hiroko, Oakdo Haruo
    • Organizer
      第38会日本神経科学学会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Exploring the original function of AMPA receptor2015

    • Author(s)
      Hirai,S., Hotta,K.,Kubo,Y., Okamura,Y., Okado,H.
    • Organizer
      第92回日本生理学会
    • Place of Presentation
      神戸ポートピアアイランド(兵庫県、神戸)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Remarks] 医学研HP

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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