Development of targeting therapy for sporadic ALS using RNA aptamers
| Project/Area Number |
26640036
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Kwak Shin 東京大学, 医学(系)研究科(研究院), 研究員 (40160981)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 脳神経疾患 / 神経科学 / 核酸 |
|---|
| Outline of Final Research Achievements |
Expression of abnormally calcium-permeable AMPA receptors that have Q/R site-unedited GluA2 subunit in their subunit assembly in the motor neurons is involved in the pathogenesis of sporadic ALS. We explored the possibility of stable RNA aptamers that effectively blocked Ca2+-permeable AMPA receptors as a drug for ALS using a mechanistic ALS mouse model. Using a subcutaneously placed mini-pump, the RNA aptamer was continuously infused intracerebroventricularly through an indwelling cannula. The RNA aptamer was safely delivered to brains and spinal cords without extensive degradation and effectively blocked abnormal Ca2+ influx into the cortical and spinal cord neurons. AMPA receptor-specific RNA aptamers may be a potential drug for ALS.
|
Report
(3 results)
Research Products
(31 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] ALS 治療への展望2014
Author(s)
郭 伸
Organizer
第12回神経難病とケアを考える会,
Place of Presentation
エーザイホール, 東京
Year and Date
2014-06-28
Related Report
Invited
-
-
-
[Presentation] 子宮内電気穿孔法TDP-43遺伝子導入によるin vivo形成封入体の微細形態の検討2014
Author(s)
詫間 浩, 赤松 恵, 山下雄也, Hartmut Oehring, 岡田拓也, 枡 和子, 石井一弘, Gustav Jirikowsk, 郭 伸, 枡 正幸, 玉岡 晃
Organizer
第55回日本神経学会学術大会
Place of Presentation
福岡国際会議場, 福岡
Year and Date
2014-05-21 – 2014-05-24
Related Report
-
-
-
-
