Spatiotemporal expression pattern of splice variants of synapse organizers
| Project/Area Number |
26640038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | University of Toyama |
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Principal Investigator |
Yoshida Tomoyuki 富山大学, 大学院医学薬学研究部(医学), 准教授 (90372367)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 選択的スプライシング / シナプスオーガナイザー / スプライシング / シナプス形成 |
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| Outline of Final Research Achievements |
Synapse formation in the brain is partly mediated by a small subset of trans-synaptic cell adhesion molecules called synapse organizers. We found that PTPδ one of presynaptic synapse organizers, existed in multiple splice variants generated by insertions of 3, 6 and 4 amino-acid residue peptides (termed mini-exon peptides) in the extracellular immunoglobulin (Ig)-like domains. At least 8 splice variants in the Ig-like domains of PTPδ were expressed in the developing mouse brains. Proportions of these splice variants varied across brain regions and developmental stages. Furthermore, structural analyses of synapse-organizing complex of PTPδ and its ligands revealed the role of mini-exon peptides on the ligand recognition and synaptic differentiation. These results suggest that the choice of mini-exons may be determinants for selective pairing between PTPδ variants and their corresponding postsynaptic ligands to ensure the target specific synapse formation in the brain.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Structure of Slitrk2-PTPδ complex reveals mechanisms for splicing-dependent trans-synaptic adhesion2015
Author(s)
Yamagata A., Sato, Y., Goto-Ito, S., Uemura, T., Maeda, A., Shiroshima, T., Yoshida, T., Fukai , S.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 9686-9686
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation2015
Author(s)
Yamagata A., Yoshida, T., Sato, Y., Goto-Ito, S., Uemura, T., Maeda, A., Shiroshima, T., Iwasawa-Okamoto, S., Mori, H., Mishina, M., Fukai , S.
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 6926-6926
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] IL1RAPL1 knockout mice show learning deficiency, memory decline, hyperactivity and reduced anxiety-like behaviours.2014
Author(s)
Misato Yasumura, Tomoyuki Yoshida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kouta Kanno, Takeshi Uemura, Keizo Takao, Kenji Sakimura, Takefumi Kikusui, Tsuyoshi Miyakawa, Masayoshi Mishina
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Journal Title
Scientific Reports
Volume: 4: 6613
Issue: 1
Pages: 6613-6613
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A590T mutation in KCNQ1 C-terminal helix D decreases IKs channel trafficking and function but not Yotiao interaction.2014
Author(s)
Kinoshita K, Komatsu T, Nishide K, Hata Y, Hisajima N, Takahashi H, Kimoto K, Aonuma K, Tushima E, Tabata T, Yoshida T, Mori H, Nishida K, Yamaguchi Y, Ichida F, Fukurotani K, Inoue H, Nishida N.
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Journal Title
J Mol Cell Cardiol
Volume: 72
Pages: 273-280
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 小脳シナプス形成を制御するGluD2-Cbln1-neurexin接着分子複合体の構造基盤2015
Author(s)
植村健, 佐藤祐介, 山形敦史, 吉田知之, 後藤桜子, 前田亜沙美, 城島知子, 田渕克彦, 三品昌美, 深井周也.
Organizer
第38回日本分子生物学会年会 ワークショップ.
Place of Presentation
神戸国際会議場, 神戸
Year and Date
2015-12-01
Related Report
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[Presentation] シナプス形成を司るIL1RAPL1-PTPδ複合体の構造基盤2015
Author(s)
吉田知之, 山形敦史, 佐藤祐介, 伊藤桜子, 植村健, 前田亜沙美, 城島知子, 岡本志穂, 森寿, 三品昌美, 深井周也.
Organizer
第38回日本神経科学大会
Place of Presentation
神戸国際会議場, 神戸
Year and Date
2015-07-31
Related Report
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