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Analyses of dynamic movement of intracellular structures underlying neuronal migration in the developing brain

Research Project

Project/Area Number 26640040
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionChubu University

Principal Investigator

SAKAKIBARA Akira  中部大学, 生命健康科学部, 准教授 (20510217)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞移動 / ニューロン / 微小管 / アクチン / ニューロン移動 / イメージング
Outline of Final Research Achievements

Neuronal migration is an integrative process involving leading process formation, nucleokinesis, and rear retraction, all of which depend on the function of cytoskeletons. In this study, live imaging of microtubule (MT) and actin cytoskeletons in migrating neocortical neurons in slice culture was performed. In migrating neurons, two types of nucleokinesis were observed. The first one is the nucleus-centrosome (N-C) coupling type of nucleokinesis. Centrosome proceeded into the leading process prior to the N-C coupling type of nucleokinesis. Rear accumulation of F-actin was not necessarily observed during the N-C coupling type of nucleokinesis. The second type of nucleokinesis is the N-C inversion during which translocating nucleus overtake the centrosome. Rear accumulations of F-actin were observed when the N-C inversion occurred. These data suggest that the actomyosin system underlies the N-C inversion type of nucleokinesis during which the MT-dynein system could not pull the nucleus.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] ミュンスター大学(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Rap1 GTPases are master regulators of neural cell polarity in the developing neocortex2016

    • Author(s)
      Bhavin Shah
    • Journal Title

      Cereb. Cortex

      Volume: 印刷中 Pages: bhv341-bhv341

    • DOI

      10.1093/cercor/bhv341

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Neuronal polarization in the developing cerebral cortex2015

    • Author(s)
      Akira Sakakibara
    • Journal Title

      Front. Neurosci.

      Volume: 9 Pages: 116-116

    • DOI

      10.3389/fnins.2015.00116

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Rear accumulation of actin underlies nucleus-centrosome inversion type of nucleokinesis in migrating neocortical neurons2015

    • Author(s)
      Akira Sakakibara
    • Organizer
      EMBO2015
    • Place of Presentation
      Birmingham(英国)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rear accumulation of actin underlies nucleus-centrosome inversion type of nucleokinesis in migrating neocortical neurons2014

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 2014 ASCB/IFCB meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-07 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 大脳皮質ニューロンの移動と極性化における微小管の機能2014

    • Author(s)
      榊原明
    • Organizer
      第66回 日本細胞生物学会大会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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