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Axon regeneration in the central nervous system with a novel blocker for semaphorin

Research Project

Project/Area Number 26640043
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionUniversity of Hyogo (2016)
Kyoto University (2014-2015)

Principal Investigator

Oinuma Izumi  兵庫県立大学, 生命理学研究科, 教授 (40452297)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsaxon / small GTPase / R-Ras / axon regeneration / actin / 神経再生 / 低分子量Gタンパク質 / アクチン細胞骨格 / 選択的スプライシング / ガイダンス因子 / 再生医学 / 神経科学 / ガイダンス分子 / アクチン / afadin / 軸索
Outline of Final Research Achievements

We have reported that GTP-bound R-Ras stimulates axonal elongation and branching in cultured neurons. Elucidation of regulatory mechanisms for axonal morphogenesis by active R-Ras is helpful for axonal regenerative study, and we searched for R-Ras-binding proteins responsible for axonal development through yeast-two-hybrid screening. Through the screening, we identified an actin-binding protein, afadin, for R-Ras binding partner mediating axonal branching. We further identified alternative variants for afadin, l-afadin and s-afadin, and their opposite roles for inducing axonal branching.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Signaling mechanism for axonal guidance factors, semaphorins2015

    • Author(s)
      生沼泉
    • Journal Title

      生化学

      Volume: 87 Issue: 4 Pages: 428-437

    • DOI

      10.14952/SEIKAGAKU.2015.870428

    • NAID

      40020571816

    • ISSN
      0037-1017
    • Year and Date
      2015-08-25
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A short splicing isoform of afadin suppresses the cortical axon branching in a dominant-negative manner2015

    • Author(s)
      Umeda K, Iwasawa N, Negishi M, Oinuma I
    • Journal Title

      Molecular Biology of the Cell

      Volume: 26 Issue: 10 Pages: 1957-1970

    • DOI

      10.1091/mbc.e15-01-0039

    • NAID

      120005997939

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A short splicing isoform of afadin suppresses the cortical axon branching in a dominant-negative manner.2015

    • Author(s)
      Kentaro Umeda, Nariaki Iwasawa, Manabu Negishi, and Izumi Oinuma
    • Journal Title

      Molecular Biology of the Cell

      Volume: 26

    • NAID

      120005997939

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Live-cell imaging and functional analysis of endogenous neuronal proteins by CRISPR/Cas9-mediated genome editing.2016

    • Author(s)
      Izumi Oinuma
    • Organizer
      平成28年度日本生化学会大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 軸索形態調節におけるR-Rasサブファミリー低分子量Gタンパク質の役割2016

    • Author(s)
      生沼泉、根岸学
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] R-Rasを介した神経細胞の軸索の枝分かれ制御におけるafadinの2つのアイソフォーム、l-afadinとs-afadinの機能2014

    • Author(s)
      梅田健太郎、生沼泉、根岸学
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 神経軸索ガイダンス分子セマフォリンの情報伝達機構2014

    • Author(s)
      生沼泉
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Opposite regulation of two afadin isoforms, l-afadin and s-afadin, of R-Ras-mediated axon branching in cultured cortical neurons2014

    • Author(s)
      梅田健太郎、生沼泉、根岸学
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Book] Semaphorin Receptors and Their Signaling2015

    • Author(s)
      Manabu Negishi, Izumi Oinuma
    • Total Pages
      17
    • Publisher
      Springer Japan
    • Related Report
      2015 Research-status Report
  • [Remarks] 所属研究機関が作成したwebページ

    • URL

      http://www.sci.u-hyogo.ac.jp/edu/kenkyuu/base25.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究者が作成したwebページ

    • URL

      http://homepage1.canvas.ne.jp/izumi/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究者個人ホームページ

    • URL

      http://homepage1.canvas.ne.jp/izumi/

    • Related Report
      2015 Research-status Report
  • [Remarks] 所属分野のホームページ

    • URL

      http://www.negishi.lif.kyoto-u.ac.jp/j/toppu.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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