| Project/Area Number |
26640051
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kubo Takeo 東京大学, 大学院理学系研究科(理学部), 教授 (10201469)
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| Research Collaborator |
SHIRAHIGE Katsuhiko 東京大学, 分子細胞生物学研究所, 教授 (90273854)
KATOU Yuki 東京大学, 分子細胞生物学研究所, 助教 (50391917)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アフリカツメガエル / 器官再生 / 未分化増殖細胞 / インターロイキン-11 / ゲノム編集 / PhyH-like / Phyhd1 / T細胞活性化 / 再生芽 / 増殖細胞 / Interleukin 11 / 脊索 / pentraxin / 再生 / 免疫細胞 / 非自己 / Interleukin / T細胞 / 自己免疫応答 / interleukin-11 / keratin-18 |
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| Outline of Final Research Achievements |
Xenopus laevis tadpoles transiently lose their tail regenerative ability during the ‘refractory period’, probably because immature leukocytes attack proliferating tail blastemal cells as ‘non-self’ during the period. In the present study, we aimed to clarify molecular mechanisms underlying tail regeneration through functional analyses of interleukin-11 (il-11), which is selectively expressed in the proliferating tail blastemal cells, and XPhyh-like, which is a putative marker gene for leukocytes that attack tail blastemal cells.
The results indicated that il-11 is necessary for tail regeneration and play important roles in the induction and maintenance of undifferentiated cells of various tissue origin in tail blastema. In addition, XPhyH-like was suggested to be related to impaired tail regenerative ability caused by immature leukocytes, because murine XPhyH-like homologue (Phyhd1) was induced in leukocytes accompanied with the leukocyte activation.
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