| Project/Area Number |
26640053
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Itoh Yasushi 滋賀医科大学, 医学部, 准教授 (90324566)
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| Co-Investigator(Kenkyū-buntansha) |
土屋 英明 滋賀医科大学, 動物生命科学研究センター, 技術専門職員 (10378440)
佐々木 えりか 公益財団法人実験動物中央研究所, 応用発生学研究センター, センター長 (70390739)
依馬 正次 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
小笠原 一誠 滋賀医科大学, 医学部, 教授 (20169163)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 老化 / 癌 / トランスレーショナルリサーチ / 病理学 / 霊長類モデル / ゲノム編集 / レンチウイルスベクター / 遺伝子編集 |
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| Outline of Final Research Achievements |
We established genetically modified cynomolgus monkeys to reveal pathogenicity of a premature aging syndrome. Using a lentivirus vector, we obtained cynomolgus macaques that express GFP in the whole body. Next, we made plasmid vectors to examine gene modification in the cultured cells of cynomolgus macaques using CRISPR/Cas9 genome editing technology. We confirmed the high efficiency of genome editing in the Werner DNA helicase gene with the CRISPR/Cas9 system in vitro. Thereafter, using the CRISPR/Cas9 system and in vitro microfertilization, we have obtained a cynomolgus macaque that carries gene mutation in the Werner DNA helices gene. We continue observation of this macaque and examination of disease pathogenesis.
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