| Project/Area Number |
26640055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TACHIBANA Makoto 徳島大学, 疾患酵素学研究センター, 教授 (80303915)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 性決定遺伝子 |
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| Outline of Final Research Achievements |
In XY mammal, Sex-determining region Y (Sry) transiently expresses in undifferentiated embryonic gonads, thereby inducing testis differentiation. I tried to establish antibodies that recognize endogenous mouse SRY protein to identify SRY target genes. Gonads from mouse embryos at embryonic day 11.5 were subjected to ChIP analysis with the anti-SRY antibodies. Next, ChIPed DNA was applied to the next generation sequencing analysis to identify candidates of SRY target genes.
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