What is the authentic tumor initiating cells in healthy mice?

• Project/Area Number: 26640066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Hokkaido University
• Principal Investigator: Wada Haruka 北海道大学, 遺伝子病制御研究所, 講師 (70392181)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 造腫瘍細胞 / がん幹細胞 / 免疫 / がん細胞の特性 / 免疫細胞 / 造腫瘍性 / 造腫瘍能 / 相互作用

Outline of Final Research Achievements

Many kinds of tumor cells include in tumor tissue. Recent research using immune-deficient mice revealed that tumor initiating cells (TIC) only have a potential to form tumor tissue. However, we proposed that we need to identify TIC in immune-healthy body. Such TIC is authentic TIC which we should realistically focused on. We found that TIC secretes TIC-associated immune cell factors (TICIF). TICIF deficient TIC no longer initiated tumor in immune-healthy body but they nevertheless formed tumor in immune-deficient mice. These results indicated that TIC secreting tumor cells are authentic tumor cells in immune-healthy body.

Research Products

• [Journal Article] Identification of a Highly Immunogenic Mouse Breast Cancer Sub Cell Line, 4T1-S. (2016)
  • Author(s): Abe H, Wada H, Baghdadi M, Nakanishi S, Usui Y, Tsuchikawa T, Shichinohe T, Hirano S and Seino K.
  • Journal Title: Human Cell Volume: 29(2) Issue: 2 Pages: 58-66
  • DOI: 10.1007/s13577-015-0127-1
  • NAID: 120006024339
  • Peer Reviewed
• [Journal Article] α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell (2015)
  • Author(s): Hayashi E, Hachiya K, Kojo S, Baghdadi M, Takeuchi S, Yamanaka H, Abe H, Wada H and Seino K.
  • Journal Title: Inflammation and Regeneration Volume: 35 Issue: 5 Pages: 244-254
  • DOI: 10.2492/inflammregen.35.244
  • NAID: 130005114587
  • ISSN: 1880-8190, 1880-9693
  • Peer Reviewed
• [Journal Article] Myeloid molecular characteristics of human γδT cells support their acquisition of tumor antigen-presenting capacity. (2015)
  • Author(s): Muto M, Baghdadi M, Maekawa R, Wada H, and Seino K.
  • Journal Title: Cancer Immunology, Immunotherapy Volume: 64(8) Issue: 8 Pages: 941-949
  • DOI: 10.1007/s00262-015-1700-x
  • NAID: 120005820703
  • Peer Reviewed
• [Journal Article] Chemotherapy-derived inflammatory responses accelerate the formation of immunosuppressive myeloid cells in the tissue microenvironment of human pancreatic cancer. (2015)
  • Author(s): Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Shichinohe T, Takano H, Hirano S, and Seino K.
  • Journal Title: Cancer Res. Volume: 75(13), Issue: 13 Pages: 2629-4260
  • DOI: 10.1158/0008-5472.can-14-2921
  • NAID: 120005768773
  • Peer Reviewed
• [Journal Article] New immunosuppressive strategies for transplantation based on pluripotent stem cell (PSC)-derived immunoregulatory cells. (2015)
  • Author(s): Seino K, Wada H, Baghdadi M.
  • Journal Title: Stem Cell Transl Invest Volume: 2 Pages: 1-6
  • DOI: 10.14800/scti.504
  • Peer Reviewed / Open Access
• [Journal Article] Myeloid molecular characteristics of human γδT cells support their acquisition of tumor antigen-presenting capacity. (2015)
  • Author(s): Muto M, Baghdadi M, Maekawa R, Wada H, and Seino K
  • Journal Title: Cancer Immunology, Immunotherapy Volume: 未定
  • NAID: 120005820703
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of Macrophage-Like Immunosuppressive Cells from Mouse ES Cells That Contribute to Prolong Allogeneic Graft Survival. (2014)
  • Author(s): Kudo H, Wada H, Sasaki H, Tsuji H, Otsuka R, Baghdadi M, Kojo S, Chikaraishi T, Seino K.
  • Journal Title: PLoS One. Volume: 9 Issue: 10 Pages: 1-9
  • DOI: 10.1371/journal.pone.0111826
  • Peer Reviewed / Open Access
• [Journal Article] Blocking monoclonal antibodies of TIM proteins as orchestrators of anti-tumor immune response. (2014)
  • Author(s): Baghdadi M, Takeuchi S, Wada H, Seino K.
  • Journal Title: mAbs (monoclonal antibodies) Volume: 6(5) Issue: 5 Pages: 1124-1132
  • DOI: 10.4161/mabs.32107
  • Peer Reviewed / Open Access
• [Presentation] IL-34 accelerates the formation of immunosuppressive macrophages in chemoresistant tumors (2015)
  • Author(s): Muhammad Baghdadi, Sayaka Nakanishi, Haruka Wada, Ken-ichiro Seino
  • Organizer: 第44回 日本免疫学会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-11-18
• [Presentation] ヒト膵癌微小環境におけるMyeloid-derived suppressor cellsの形成は化学療法により促進される (2015)
  • Author(s): 武内慎太郎、ムハンマド・バグダーディー、土川貴裕、和田はるか、中村透、七戸俊明、清野研一郎、平野聡
  • Organizer: 第95回北海道医学大会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2015-09-12
• [Presentation] Chemotherapy-induced IL-34 accelerates the formation of tumor associated macrophages (TAMs) in human tumor microenvironment (2015)
  • Author(s): Muhammad Baghdadi, Sayaka Nakanishi, Haruka Wada, Ken-ichiro Seino
  • Organizer: 日本がん免疫学会
  • Place of Presentation: 伊藤国際学術研究センター（東京都・文京区）
  • Year and Date: 2015-07-09
• [Book] 『日本臨床』増刊 再生医療－新たな医療を求めて― (2015)
  • Author(s): 和田はるか、工藤浩也、佐々木元、清野研一郎
  • Publisher: 日本臨床社
• [Remarks] 北海道大学遺伝子病制御研究所免疫生物分野 研究内容
  • URL: http://www.igm.hokudai.ac.jp/Immunobiology-Web/Research.html