Elucidating chromosomal instability by chromosome tracking in live cell imaging
| Project/Area Number |
26640067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Tohoku University |
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Principal Investigator |
Tanaka Kozo 東北大学, 加齢医学研究所, 教授 (00304452)
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| Co-Investigator(Renkei-kenkyūsha) |
ITOH GO 東北大学, 加齢医学研究所, 助教 (60607563)
IKEDA MASANORI 東北大学, 加齢医学研究所, 教育研究支援者 (80645010)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 癌 / 細胞・組織 / 染色体不安定性 |
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| Outline of Final Research Achievements |
We aimed to elucidate the mechanism of chromosomal instability commonly seen in cancer cells. We hypothesized that modest chromosomal instability that allows cell survival, not severe instability causing cell death, is involved in cancer formation and progression, and focused on the defect in efficient chromosome alignment to the spindle equator. To address the point, we developed methods to visualize single chromosome and observe its dynamics. We also found that two motor proteins, CENP-E and Kid, is involved in chromosome alignment (Nat Commun, 2015). In Kid-depleted cells, there was a delay in chromosome alignment and chromosomal instability was induced, exemplifying the occurrence of chromosomal instability by modest mitotic defects.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell.2016
Author(s)
Hasanpourghadi, M, Karthikeyan, C, Pandurangan, A, K, Looi, C, Y, Trivedi, P, Kobayashi, K, Tanaka, K, Wong W, F, Mustafa, M, R.
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Journal Title
J Exp Clin Cancer Res
Volume: 35
Issue: 1
Pages: 38-38
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A novel anti-microtubule agent with carbazole and benzohydrazide structures suppresses tumor cell growth in vivo2015
Author(s)
M. Ohira, Y. Iwasaki, C. Tanaka, M. Kuroki, N. Matsuo, T. Kitamura, M. Yukuhiro, H. Morimoto, N. Pang, B. Liu, T. Kiyono, M. Amemiya, K. Tanaka, K. Yoshida, N. Sugimoto, T. Ohshima, M. Fujita
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Journal Title
Biochimica et Biophysica Acta (BBA) - General Subjects
Volume: 1850
Issue: 9
Pages: 1676-1684
DOI
Related Report
Peer Reviewed
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[Presentation] 染色体不安定性と発がん2015
Author(s)
田中耕三
Organizer
第9回日本エピジェネティクス研究会
Place of Presentation
学術総合センター（千代田区・東京都）
Year and Date
2015-05-25
Related Report
Invited
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