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Elucidating chromosomal instability by chromosome tracking in live cell imaging

Research Project

Project/Area Number 26640067
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionTohoku University

Principal Investigator

Tanaka Kozo  東北大学, 加齢医学研究所, 教授 (00304452)

Co-Investigator(Renkei-kenkyūsha) ITOH GO  東北大学, 加齢医学研究所, 助教 (60607563)
IKEDA MASANORI  東北大学, 加齢医学研究所, 教育研究支援者 (80645010)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords癌 / 細胞・組織 / 染色体不安定性
Outline of Final Research Achievements

We aimed to elucidate the mechanism of chromosomal instability commonly seen in cancer cells. We hypothesized that modest chromosomal instability that allows cell survival, not severe instability causing cell death, is involved in cancer formation and progression, and focused on the defect in efficient chromosome alignment to the spindle equator. To address the point, we developed methods to visualize single chromosome and observe its dynamics. We also found that two motor proteins, CENP-E and Kid, is involved in chromosome alignment (Nat Commun, 2015). In Kid-depleted cells, there was a delay in chromosome alignment and chromosomal instability was induced, exemplifying the occurrence of chromosomal instability by modest mitotic defects.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (34 results)

All 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 3 results) Presentation (21 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Remarks (4 results)

  • [Int'l Joint Research] University of Malaya(マレーシア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Rajiv Gandhi Technical University(インド)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell.2016

    • Author(s)
      Hasanpourghadi, M, Karthikeyan, C, Pandurangan, A, K, Looi, C, Y, Trivedi, P, Kobayashi, K, Tanaka, K, Wong W, F, Mustafa, M, R.
    • Journal Title

      J Exp Clin Cancer Res

      Volume: 35 Issue: 1 Pages: 38-38

    • DOI

      10.1186/s13046-016-0332-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A novel anti-microtubule agent with carbazole and benzohydrazide structures suppresses tumor cell growth in vivo2015

    • Author(s)
      M. Ohira, Y. Iwasaki, C. Tanaka, M. Kuroki, N. Matsuo, T. Kitamura, M. Yukuhiro, H. Morimoto, N. Pang, B. Liu, T. Kiyono, M. Amemiya, K. Tanaka, K. Yoshida, N. Sugimoto, T. Ohshima, M. Fujita
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 1850 Issue: 9 Pages: 1676-1684

    • DOI

      10.1016/j.bbagen.2015.04.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CLIP-170 tethers kinetochores to microtubule plus ends against poleward force by dynein for stable kinetochore-microtubule attachment.2015

    • Author(s)
      Amin, M, A, Kobayashi, K, and Tanaka, K.
    • Journal Title

      FEBS Lett

      Volume: 589 Issue: 19PartB Pages: 2739-2746

    • DOI

      10.1016/j.febslet.2015.07.036

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mitosis.2015

    • Author(s)
      Tanaka, K.
    • Journal Title

      eLS

      Volume: - Pages: 1-11

    • DOI

      10.1002/9780470015902.a0001356.pub2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集 染色体安定性制御と疾患 総論 染色体不安定性の病態生理.2015

    • Author(s)
      田中耕三.
    • Journal Title

      細胞

      Volume: 47 Pages: 212-214

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Chromokinesin Kid and kinetochore kinesin CENP-E differentially support congression without end-on attachment to microtubules2015

    • Author(s)
      家村顕自、田中耕三
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 6447-6447

    • DOI

      10.1038/ncomms7447

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CLIP-170 is required to recruit PLK1 to kinetochores during early mitosis for chromosome alignment2014

    • Author(s)
      M.A.Amin、伊藤剛、家村顕自、池田真教、田中耕三
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 2818-2824

    • DOI

      10.1242/jcs.150755

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Defective chromosome dynamics as a cause of chromosomal instability in cancer2016

    • Author(s)
      田中耕三
    • Organizer
      International Conference of the Korean Society for Molecular and Cellular Biology 2016
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2016-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 効率的な染色体整列の異常による染色体不安定性の出現2016

    • Author(s)
      田中耕三
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋市・愛知県)
    • Year and Date
      2016-10-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] M期キナーゼPlk1による紡錘体チェックポイント制御機構の解明2016

    • Author(s)
      池田真教, 田中耕三
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊(松島町・宮城県)
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 効率的な染色体整列の染色体安定性への関与2016

    • Author(s)
      國安絹枝, 家村顕自, 田中耕三
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊(松島町・宮城県)
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 赤道面に整列した染色体でのキネトコア-微小管結合修正におけるAurora Aの役割2016

    • Author(s)
      家村顕自, 田中耕三
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊(松島町・宮城県)
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゲノム安定性を司る紡錘体チェックポイントの新規分子機構の解明2015

    • Author(s)
      池田真教, 田中耕三
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場(神戸市・兵庫)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 効率的な染色体整列の染色体安定性への関与2015

    • Author(s)
      國安絹枝, 家村顕自, 田中耕三
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場(神戸市・兵庫)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 効率的な染色体整列の染色体安定性への関与2015

    • Author(s)
      小林絹枝, 家村顕自, 田中耕三
    • Organizer
      平成27年度がん若手研究者ワークショップ
    • Place of Presentation
      蓼科グランドホテル滝の湯(茅野市・長野県)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 効率的な染色体整列の異常による染色体不安定性の出現2015

    • Author(s)
      田中耕三
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋市・愛知県)
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of Kid and CENP-E on efficient chromosome alignment2015

    • Author(s)
      Kenji Iemura, Kozo Tanaka
    • Organizer
      第10回研究所ネットワ-ク国際シンポジウム
    • Place of Presentation
      北海道大学医学部学友会館(札幌市・北海道)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 効率的な染色体整列におけるKid及びCENP-Eの機能解析2015

    • Author(s)
      家村顕自, 田中耕三
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(江戸川区・東京都)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 染色体不安定性と発がん2015

    • Author(s)
      田中耕三
    • Organizer
      第9回日本エピジェネティクス研究会
    • Place of Presentation
      学術総合センター(千代田区・東京都)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 効率的な染色体整列におけるKid 及びCENP-E の役割2015

    • Author(s)
      家村顕自, 田中耕三
    • Organizer
      第81回日本生化学会東北支部例会
    • Place of Presentation
      東北大学片平さくらホール(仙台市・宮城県)
    • Year and Date
      2015-05-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chromosomal instability: a road to cancer and aging2015

    • Author(s)
      Kozo Tanaka
    • Organizer
      Alfred Benzon Workshop
    • Place of Presentation
      コペンハーゲン(デンマーク)
    • Year and Date
      2015-05-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 体細胞分裂期でのM期チェックポイントを制御する新規分子機構の解明2014

    • Author(s)
      池田真教、田中耕三
    • Organizer
      第32回染色体ワークショップ・第13回核ダイナミクス研究会
    • Place of Presentation
      安芸グランドホテル(広島)
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 効率的な染色体整列におけるKid及びCENP-Eの機能解析2014

    • Author(s)
      家村顕自、水野夏紀、小林絹枝、田中耕三
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Mps1キナーゼの局在・活性制御機構と分裂期チェックポイントにおけるその機能的役割の解明2014

    • Author(s)
      池田真教、田中耕三
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] M期チェックポイントキナーゼMps1の活性調節機構とその機能的役割の解明2014

    • Author(s)
      池田真教、田中耕三
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 染色体整列制御分子CAMPは分裂期停止時におけるがん細胞の生存に寄与する2014

    • Author(s)
      家村顕自、伊藤剛、田中耕三
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 染色体恒常性の維持に必須な分裂期チェックポイント制御機構の解明2014

    • Author(s)
      池田真教
    • Organizer
      平成26年度がん若手研究者ワークショップ
    • Place of Presentation
      蓼科グランドホテル滝の湯(長野)
    • Year and Date
      2014-09-03 – 2014-09-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 動原体キネシンCENP-EとクロモキネシンKidによる染色体運動の制御2014

    • Author(s)
      田中耕三
    • Organizer
      理研シンポジウム 第4回分子モーター討論会
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Research-status Report
  • [Remarks] 東北大学加齢医学研究所

    • URL

      http://www.idac.tohoku.ac.jp/index.ja.php

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東北大学加齢医学研究所分子腫瘍学研究分野

    • URL

      http://www2.idac.tohoku.ac.jp/dep/molonc/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 田中耕三研究室 Department of Molecular Oncology

    • URL

      http://www2.idac.tohoku.ac.jp/dep/molonc/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 東北大学加齢医学研究所 分子腫瘍学研究分野

    • URL

      http://www.idac.tohoku.ac.jp/ja/activities/research/molecular_oncology/index.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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