The mechanism of "alteration to a cancer stem cell" in ovarian cancer
| Project/Area Number |
26640076
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA NORIOMI 京都大学, 医学研究科, 準教授 (20452336)
BABA TSUKASA 京都大学, 医学研究科, 講師 (60508240)
KONISHI IKUO 京都大学, 医学研究科, 教授 (90192062)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 癌幹細胞 / 化学療法耐性 / ヘッジホッグ経路 / 治療抵抗性獲得 / 卵巣癌 / SP分画 / 卵巣漿液性腺癌 / shRNA / single clonogeneisity / chemosennsitivity / sphere formation / shRNAライブラリー / ＳＰ分画 / single clonogenesity / chemosensitivity / sphere formation assay |
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| Outline of Final Research Achievements |
Ovarian cancer frequently acquires malignant phenotypes, such as chemo-resistance and tumorgenicity. We hypothesized that suppression of a gene’s expression might cause such an acquisition. We performed a functional genomics screen using a shRNA library targeting almost all genes. As a result, we found that suppression of only a single gene’s expression caused the acquisition of malignant phenotypes of ovarian cancer. In addition, we identified at least 6 such kinds of genes. Furthermore, suppression of those 6 genes enhanced malignant phenotypes of ovarian cancer via activation of the Hedgehog pathway. When we inhibited its pathway by a specific inhibitor, cyclopamine, it markedly decreased the malignant phenotypes which had been enhanced by suppression of each of 6 genes. Our findings should be helpful for developing the new treatment for refractory ovarian cancer.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Menstrual cyclic change of metastin/GPR54 in endometrium.2014
Author(s)
BabaT, Kang HS, Hosoe Y, Kharma B, Aniko K, Matsumura N, Hamanishi J, Yamaguchi K, Yoshioka Y, Koshiyama M, N\Mandai M, Murphy SK, Konishi I
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Journal Title
Med Mol Morphol
Volume: 08
Issue: 2
Pages: 1-9
DOI
Related Report
Peer Reviewed
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[Presentation] Identification through functional genomics screening of factors whose downregulation enhances the side population in ovarian cancer.2015
Author(s)
Noriomi Matsumura, Koji Yamanoi, Susan K Murphy, Junzo Hamanishi, Kaoru Abiko, Ken Yamaguchi, Tsukasa Baba, Masafumi Koshiyama, Ikuo Konishi
Organizer
An AACR Special Conference on Advances in Ovarian Cancer Research
Place of Presentation
Hyatt Regency Orland, Orland, FL
Year and Date
2015-10-17
Related Report
Int'l Joint Research / Invited
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