Elucidation of Warburg effect in cancer with the next-generation proteomics
| Project/Area Number |
26640080
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | がん / プロテオミクス / ワールブルグ効果 / 代謝 |
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| Outline of Final Research Achievements |
we developed a new platform (information-based MRM: iMRM) that allows genome-wide absolute quantification of the human proteome and is reliant on the production of ~18,000 recombinant proteins. We applied iMRM to delineate the metabolic landscape of human diploid fibroblasts. Oncogenic transformation of these cells gave rise to relatively small but global changes in metabolic pathways that account for aerobic glycolysis (Warburg effect) and increased rates of macromolecule synthesis. Modulation of metabolic enzyme expression revealed an unexpected functional interaction between glycolysis and the pentose phosphate pathway that facilitates nucleic acid synthesis. Furthermore, integration of proteomic and metabolomic data allowed construction of a mathematical model for identification of key enzymes responsible for the metabolic shift in cancer. Our results thus provide a global view of metabolic restructuring in cancer that underlies adaptation to a rapid growth state.
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Report
(2 results)
Research Products
(10 results)
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[Journal Article] Reconstruction of insulin signal flow from phosphoproteome and metabolome data2014
Author(s)
Yugi, K., Kubota, H., Toyoshima, Y., Noguchi, R., Kawata, K., Komori, Y., Uda, S., Kunida, K., Tomizawa, Y., Funato, Y., Miki, H., Matsumoto, M., Nakayama, K.I., Kashikura, K., Endo, K., Ikeda, K., Soga, T., Kuroda, S.
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Journal Title
Cell Rep.
Volume: 8
Issue: 4
Pages: 1171-1183
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway2014
Author(s)
Adachi S, Homoto M, Tanaka R, Hioki Y, Murakami H, Suga H, Matsumoto M, Nakayama KI, Hatta T, Iemura S, Natsume T.
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Journal Title
Nucleic Acids Research
Volume: 42
Issue: 15
Pages: 10037-10049
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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