Research Project
Grant-in-Aid for Challenging Exploratory Research
we developed a new platform (information-based MRM: iMRM) that allows genome-wide absolute quantification of the human proteome and is reliant on the production of ~18,000 recombinant proteins. We applied iMRM to delineate the metabolic landscape of human diploid fibroblasts. Oncogenic transformation of these cells gave rise to relatively small but global changes in metabolic pathways that account for aerobic glycolysis (Warburg effect) and increased rates of macromolecule synthesis. Modulation of metabolic enzyme expression revealed an unexpected functional interaction between glycolysis and the pentose phosphate pathway that facilitates nucleic acid synthesis. Furthermore, integration of proteomic and metabolomic data allowed construction of a mathematical model for identification of key enzymes responsible for the metabolic shift in cancer. Our results thus provide a global view of metabolic restructuring in cancer that underlies adaptation to a rapid growth state.
All 2015 2014 Other
All Journal Article (3 results) (of which Peer Reviewed: 3 results, Open Access: 3 results, Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Invited: 5 results) Remarks (2 results)
Cell Rep.
Volume: 8 Issue: 4 Pages: 1171-1183
10.1016/j.celrep.2014.07.021
120005537752
Molecular and Cellular Biology
Volume: 34 Issue: 17 Pages: 3321-3340
10.1128/mcb.00320-14
Nucleic Acids Research
Volume: 42 Issue: 15 Pages: 10037-10049
10.1093/nar/gku652
http://www.bioreg.kyushu-u.ac.jp/saibou/index.html
http://www.bioreg.kyushu-u.ac.jp/saibou/index_en.html
