| Project/Area Number |
26640086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
Hirota Toru 公益財団法人がん研究会, がん研究所・実験病理部, 部長 (50421368)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞分裂 / 染色体分配 / 姉妹染色分体 / コヒージョン / カテネーション / コヒーシン / コンデンシン / トポイソメラーゼ IIα / 染色体構築 / トポイソメラーゼ２ / 顕微鏡的定量解析 / Ｍ期 |
|---|
| Outline of Final Research Achievements |
The formation of mitotic chromosomes requires both compaction of chromatin and the resolution of replicated sister chromatids. Compaction occurs during prophase, depending on the activity of condensin II. Exactly when sister chromatid resolution occurs has been largely unknown, as has its molecular requirements. In this study, we visualized sister resolution by sequential replication labelling with two distinct nucleotide derivatives. Quantitative analyses allowed us to find that sister resolution starts already at the beginning of prophase, proceeds concomitantly with chromatin compaction and is largely completed by the end of prophase. Sister resolution was abolished by inhibition of topoisomerase IIα and by inactivation of condensin II, whereas blocking cohesin dissociation from chromosomes had little effect. Sister resolution is thus an intrinsic part of mitotic chromosome formation in prophase, and shares the molecular requirement for condensin II with prophase compaction.
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