Development of in vitro assay system for carcinogenesis using iPS-derived intestinal epithelial stem cells
Project/Area Number |
26640089
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
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Research Institution | National Cancer Center Japan |
Principal Investigator |
Mutoh Michihiro 国立研究開発法人国立がん研究センター, 社会と健康研究センター, 室長 (30392335)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 大腸がん / iPS / 放射線照射 / 上皮間葉変換 / iPS細胞 / がん予防 / 発がん事象 / 3次元細胞培養 |
Outline of Final Research Achievements |
<Aim> The processes of carcinogenesis are so complex that they are difficult to reproduce in a cell system. However, establishment of a simple model may accelerate our knowledge of carcinogenetic processes. In the present study, we tried to reproduce carcinogenetic processes by the induction of stochastic changes in vitro. <Methods & Results> We first obtained stem cells from the lower digestive tract derived from iPS cells. The maintenance medium allowed us to culture the cells for more than a half-year without any neoplastic feature changes. We next tried to induce somatic mutation by treating 53-passage-cells with 5 Gy radiation. Five months later, the cells were obviously changed in shape to spindle formation. The molecular markers of differentiation and intestinal epithelium were down-regulated, and we obtained epithelial-mesenchymal transformed cells. <Future plan> We are now evaluating the neoplastic characters using a xenograft model.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in <i>Apc</i><sup>Min/+</sup> mice2014
Author(s)
Shimizu S, Fujii G, Takahashi M, Nakanaishi R, Komiya M, Shimura M, Noma N, Onuma W, Yano T, Mutoh M.
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 54
Issue: 2
Pages: 95-101
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed
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[Journal Article] Association of pancreatic fatty infiltration with pancreatic ductal adenocarcinoma.2014
Author(s)
Hori M, Takahashi M, Hiraoka N, Yamaji T, Mutoh M, Ishigamori R, Furuta K, Okusaka T, Shimada K, Kosuge T, Kanai Y, Nakagama H
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Journal Title
Clinical and Translational Gastroenterology
Volume: 5
Issue: 3
Pages: e53-e53
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The preventive effects of low-dose enteric-coated aspirin tablets on the development of colorectal tumours in Asian patients: a randomised trial.2013
Author(s)
Ishikawa H, Mutoh M, Suzuki S, Tokudome S, Saida Y, Abe T, Okamura S, Tajika M, Joh T, Tanaka S, Kudo SE, Matsuda T, Iimuro M, Yukawa T, Takayama T, Sato Y, Lee K, Kitamura S, Mizuno M, Sano Y, Gondo N, Sugimoto K, Kusunoki M, Goto C, Matsuura N, Sakai T, Wakabayashi K.
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Journal Title
Gut.
Volume: Jan 31
Issue: 11
Pages: 1755-1759
DOI
Related Report
Peer Reviewed
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