Establishment of system for direct detection of exosome

• Project/Area Number: 26640091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Hiroshima University
• Principal Investigator: Tahara Hidetoshi 広島大学, 医歯薬保健学研究院(薬), 教授 (00271065)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞外小胞 / エクソソーム / miRNA / マイクロRNA / FACS

Outline of Final Research Achievements

Exosome is extracellular nano-vesicles secreted from different type of cells. However, there is no significant flow-based instrument to detect exosome because of high background noise under 500nm size detection by current flow cytometers. To overcome this problem, we used a spectral cell analyzer, SP6800 (Sony corporation) which adopts a new method to measure the full spectrum of fluorescence with a high degree of accuracy. We purified exosome and stained with Cellview reagent, and significant and specific population is detected very small size population, which is distinguished from noise population. We also found that Alexa488 labeled CD9 and Alexa647 labeled CD63 were presented in purified exosomes, but exosomes are heterogeneous population. We also tried to detect cancer specific exosome derived from pancreatic cancer, and the spectral cell analyzer SP6800 can be used to detect exosome particle directly.

Research Products

• [Journal Article] MicroRNA-203 Suppresses Invasion and Epithelial-mesenchymal Transition Induction via Targeting NUAK1 and SNAI2 in Head and Neck Cancer (2016)
  • Author(s): Obayashi, M., Yoshida, M., Tsunematsu, T., Ogawa, I., Sasahira, T., Kuniyasu, H., Imoto, I., Abiko, Y., Xu, D., Fukunaga, S., Tahara, H., Kudo, Y., Nagao, T., Takata, T.
  • Journal Title: Oncotarget Volume: 6972 Issue: 7 Pages: 8223-39
  • DOI: 10.18632/oncotarget.6972
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mutations in the gene encoding the E2 conjugating enzyme UBE2T cause Fanconi anemia. (2015)
  • Author(s): Hira A, Yoshida K, Sato K, Okuno Y, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Shimamoto A, Tahara H, Ito E, Kojima S, Kurumizaka H, Ogawa S, Takata M, Yabe H, Yabe M.
  • Journal Title: Am J Hum Genet. Volume: 96(6) Issue: 6 Pages: 1001-7
  • DOI: 10.1016/j.ajhg.2015.04.022
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Telomere G-tail Length is a Promising Biomarker Related to White Matter Lesions and Endothelial Dysfunction in Patients With Cardiovascular Risk: A Cross-sectional Study. (2015)
  • Author(s): Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M, Tahara H.
  • Journal Title: EBioMedicine. Volume: 2(8) Issue: 8 Pages: 958-65
  • DOI: 10.1016/j.ebiom.2015.05.025
  • NAID: 120006796554
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Werner Syndrome-specific induced pluripotent stem cells: recovery of telomere function by reprogramming. (2015)
  • Author(s): Shimamoto, A., Yokote, K. & Tahara, H.
  • Journal Title: Frontiers in genetics Volume: 6 Pages: 10-10
  • DOI: 10.3389/fgene.2015.00010
  • Peer Reviewed / Open Access
• [Journal Article] A potential function for neuronal exosomes: sequestering intracerebral amyloid-beta peptide. (2015)
  • Author(s): Yuyama, K., Sun, H., Usuki, S., Sakai, S., Hanamatsu, H., Mioka, T., Kimura, N., Okada, M., Tahara, H., Furukawa, J., Fujitani, N., Shinohara, Y. & Igarashi, Y.
  • Journal Title: FEBS letters Volume: 589 Issue: 1 Pages: 84-88
  • DOI: 10.1016/j.febslet.2014.11.027
  • NAID: 120005537475
  • Peer Reviewed / Open Access
• [Journal Article] Decreased amyloid-beta pathologies by intracerebral loading of glycosphingolipid-enriched exosomes in Alzheimer model mice. (2014)
  • Author(s): Yuyama, K., Sun, H., Sakai, S., Mitsutake, S., Okada, M., Tahara, H., Furukawa, J., Fujitani, N., Shinohara, Y. & Igarashi, Y.
  • Journal Title: J Biol Chem Volume: 289 Issue: 35 Pages: 24488-24498
  • DOI: 10.1074/jbc.m114.577213
  • Peer Reviewed / Open Access
• [Journal Article] Generation of Human induced pluripotent Stem (iPS) cells in serum- and feeder-free defined culture and TGF-β1 regulation of pluripotency (2014)
  • Author(s): Yamasaki S, Taguchi Y, Shimamoto A, Mukasa H, Tahara H, Okamoto T.
  • Journal Title: PLoS One Volume: 9(1) Issue: 1 Pages: 1-13
  • DOI: 10.1371/journal.pone.0087151
  • Peer Reviewed / Open Access
• [Journal Article] Reprogramming suppresses premature senescence phenotypes of Werner syndrome cells and maintains chromosomal stability over long-term culture (2014)
  • Author(s): Shimamoto A, Kagawa H, Zensho K, Sera Y, Kazuki Y, Osaki M, Oshimura M, Ishigaki Y, Hmasaki K, Kodama Y, Yuasa S, Fukuda K, Hirashima K, Seimiya H, Koyama H, Shimizu T, Takemoto M, Yokote K, Goto M, Tahara H.
  • Journal Title: PLOS ONE Volume: 9 Issue: 11 Pages: 1-13
  • DOI: 10.1371/journal.pone.0112900
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DSE-FRET : A new anticancer drug screening assay for DNA binding proteins (2014)
  • Author(s): Miyagi T, Shiotani B, Miyoshi R, Yamamoto T, Oka T, Umezawa K, Ochiya T, Takano M, Tahara H
  • Journal Title: Cancer Sci Volume: (in press) Issue: 7 Pages: 870-874
  • DOI: 10.1111/cas.12420
  • Peer Reviewed / Open Access
• [Journal Article] Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. (2014)
  • Author(s): Lotvall, J., Hill, A.F., Hochberg, F., Buzas, E.I., Di Vizio, D., Gardiner, C., Gho, Y.S., Kurochkin, I.V., Mathivanan, S., Quesenberry, P., Sahoo, S., Tahara, H., Wauben, M.H., Witwer, K.W. & Thery, C.
  • Journal Title: Journal of extracellular vesicles Volume: 3 Issue: 1 Pages: 26913-26913
  • DOI: 10.3402/jev.v3.26913
  • Peer Reviewed / Open Access
• [Journal Article] EVpedia: a community web portal for extracellular vesicles research. (2014)
  • Author(s): Kim, D.K., Lee, J., Kim, S.R., Choi, D.S., Yoon, Y.J., Kim, J.H., Go, G., Nhung, D., Hong, K., Jang, S.C., Kim, S.H., Park, K.S., Kim, O.Y., Park, H.T., Seo, J.H., Aikawa, E., Baj-Krzyworzeka, M., van Balkom, B.W., Belting, M., Blanc, L.,& Tahara, H.
  • Journal Title: Bioinformatics Volume: 31 (6) Pages: 933-939
  • Peer Reviewed
• [Journal Article] TERT attenuated ER stress-induced cell death. (2014)
  • Author(s): Hosoi, T., Inoue, Y., Nakatsu, K., Matsushima, N., Kiyose, N., Shimamoto, A., Tahara, H. & Ozawa, K.
  • Journal Title: Biochem Biophys Res Commun Volume: 447 Issue: 2 Pages: 378-382
  • DOI: 10.1016/j.bbrc.2014.04.034
  • Peer Reviewed / Open Access
• [Journal Article] CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. (2014)
  • Author(s): Hirokawa T, Shiotani B, Shimada M, Murata K, Johmura Y, Haruta M, Tahara H, Takeyama H, Nakanishi M.
  • Journal Title: Cancer Res. Volume: 74 Issue: 14 Pages: 3880-9
  • DOI: 10.1158/0008-5472.can-13-3604
  • Peer Reviewed / Open Access
• [Journal Article] Telomeric g-tail length and hospitalization for cardiovascular events in hemodialysis patients. (2014)
  • Author(s): Hirashio, S., Nakashima, A., Doi, S., Anno, K., Aoki, E., Shimamoto, A., Yorioka, N., Kohno, N., Masaki, T. & Tahara, H.
  • Journal Title: Clinical journal of the American Society of Nephrology Volume: CJASN 9 Issue: 12 Pages: 2117-2122
  • DOI: 10.2215/cjn.10010913
  • Peer Reviewed / Open Access
• [Journal Article] Infection with Porphyromonas gingivalis exacerbates endothelial injury in obese mice. (2014)
  • Author(s): Ao, M., Miyauchi, M., Inubushi, T., Kitagawa, M., Furusho, H., Ando, T., Ayuningtyas, N.F., Nagasaki, A., Ishihara, K., Tahara, H., Kozai, K. & Takata, T.
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: e110519-e110519
  • DOI: 10.1371/journal.pone.0110519
  • Peer Reviewed / Open Access
• [Presentation] 細胞外小胞・マイクロRNA研究の最前線 (2016)
  • Author(s): 田原 栄俊
  • Organizer: 一丸ファルコス株式会社講演
  • Place of Presentation: 岐阜市
  • Year and Date: 2016-03-25
  • Invited
• [Presentation] マイクロRNAを用いた抗がん剤の開発とコンパニオン診断 (2016)
  • Author(s): 田原 栄俊
  • Organizer: 最新癌治療セミナー2016
  • Place of Presentation: 広島市
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] "Non-coding RNAs Senescence-associated EVs from fibroblasts act as tumor suppressor at the tumor microenvironment." (2016)
  • Author(s): Hidetoshi Tahara
  • Organizer: Tenth AACR-JCA Joint Conference on Breakthroughs in Cancer Research
  • Place of Presentation: Maui, USA
  • Year and Date: 2016-02-18
  • Int'l Joint Research
• [Presentation] 膵臓がんの抑制と悪性化に関わる miR-486 の機能 (2015)
  • Author(s): 福永 早央里,山本 佑樹,木根原 匡希,村岡 賢,嶋本 顕,田原 栄俊,
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-03
• [Presentation] Depletion of PRPF19 regulates cell fate between cellular senescence and cell death (2015)
  • Author(s): 矢野 公義,福永 早央里,高木 翔太,塩谷 文章,木根原 匡希,村岡 賢,嶋本 顕,田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-03
• [Presentation] 疾患特異的多能性幹細胞を用いた早老症ウェルナー症候群の早期老化発症機序の解明 (2015)
  • Author(s): 嶋本 顕,加藤 尚也,横手 幸太郎,田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-02
• [Presentation] 細胞老化誘導 miRNA の網羅的探索 (2015)
  • Author(s): 山本 佑樹,高木 翔太,福永 早央里,木根原 匡希,村岡 賢,嶋本 顕,田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-02
• [Presentation] Histone variant H3.3 depletion induces cellular senescence in human diploid fibroblasts (2015)
  • Author(s): 木根原 匡希,蔵元 達谷,山本 佑樹,村岡 賢,嶋本 顕,田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-02
• [Presentation] 新規老化誘導microRNAはヒト膵癌細胞株の細胞死を誘導する (2015)
  • Author(s): 高木 翔太,福永 早央里,山本 祐樹,木根原 匡希,村岡 賢,嶋本 顕,田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-02
• [Presentation] マイクロRNA・エクソソームを用いた膵がんバイオマーカー (2015)
  • Author(s): 田原 栄俊
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-01
• [Presentation] がん微小環境における細胞外小胞エクソソームの機能 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 癌と骨病変研究会
  • Place of Presentation: 東京都
  • Year and Date: 2015-11-13
  • Invited
• [Presentation] 細胞外小胞・マイクロRNA研究の現状と展望 (2015)
  • Author(s): 田原 栄俊
  • Organizer: アステラス製薬株式会社講演会
  • Place of Presentation: つくば市
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] miRNAが医療を変える (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第14期 バイオファイナンスギルド 第4回セミナー
  • Place of Presentation: 東京都
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] マイクロRNAによる疾患予防と超早期判断 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 情報計算化学生物学会
  • Place of Presentation: 大阪市
  • Year and Date: 2015-11-05
  • Invited
• [Presentation] 膵臓がんにおける miR-486 の機能の解明 (2015)
  • Author(s): 福永 早央里,山本 佑樹,木根原 匡希,村岡 賢,嶋本 顕,田原 栄俊
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-10-10
• [Presentation] PRPF19をノックダウンしたことによる細胞老化誘導と細胞死誘導の制御機構の解明 (2015)
  • Author(s): 矢野公義,福永早央里,塩谷文章,木根原匡希,村岡賢,嶋本顕,田原栄俊
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-10-10
• [Presentation] 新規標的分子としてのがん促進的な転写因子の探索 (2015)
  • Author(s): 城間喜智,村岡賢,嶋本顕,田原栄俊
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-10-10
• [Presentation] 正常細胞における新規老化関連microRNAの同定 (2015)
  • Author(s): 山本佑樹,福永早央里木根原匡希,村岡賢,嶋本顕,田原栄俊
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-10-08
• [Presentation] エクソソーム研究の最前線ーその基礎から臨床応用まで (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第34回日本認知症学会学術集会
  • Place of Presentation: 青森市
  • Year and Date: 2015-10-02
  • Invited
• [Presentation] テロメア・マイクロRNAを用いた体液診断 (2015)
  • Author(s): 田原 栄俊
  • Organizer: JASIS2015
  • Place of Presentation: 幕張市
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] 新規核酸医薬としての miR-486 の可能性 (2015)
  • Author(s): 福永早央里,山本佑樹,木根原匡希,村岡賢,嶋本顕,田原栄俊
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2015-07-26
• [Presentation] 新規老化誘導マイクロ RNA による膵癌細胞株 の増殖抑制 (2015)
  • Author(s): 高木翔太,福永早央里,山本佑樹,木根原匡希,村岡賢,嶋本顕,田原 栄俊
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2015-07-26
• [Presentation] PRPF19 siRNA を用いた抗腫瘍効果の機構解明 (2015)
  • Author(s): 矢野公義,福永早央里,高木翔太,塩谷文章,木根原匡希,村岡賢, 嶋本顕,田原栄俊
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2015-07-26
• [Presentation] 正常細胞由来細胞外小胞は細胞老化機構を制御している (2015)
  • Author(s): 佐古直紀,村岡賢,木根原匡希,岡田恵,嶋本顕,田原栄俊
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2015-07-26
• [Presentation] 細胞老化における細胞外小胞分泌の生物学的意義の探索 (2015)
  • Author(s): 岡田恵,村岡賢,寺西茂之,佐古直紀,木根原匡希,嶋本顕,田原栄俊
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2015-07-26
• [Presentation] プロテオミクスにおけるファージディスプレイ技術の役割 (2015)
  • Author(s): 村岡 賢,田原 栄俊
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本市
  • Year and Date: 2015-07-23
• [Presentation] 膵がんを標的とした体液診断・治療薬の開発 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第19回日本分子標的治療学会学術集会
  • Place of Presentation: 松山市
  • Year and Date: 2015-06-11
• [Presentation] DSE-FRET法を用いたテロメア結合タンパク質TRF2阻害剤の探索 (2015)
  • Author(s): 城間 喜智,山本 拓弥,田原 栄俊
  • Organizer: 第19回日本分子標的治療学会学術集会
  • Place of Presentation: 松山市
  • Year and Date: 2015-06-11
• [Presentation] PKCとERK経路の二重阻害によるヒト多能性幹細胞の安定維持培養 (2015)
  • Author(s): 木根原 匡希, 田原 栄俊, 古江-楠田 美保
  • Organizer: 日本組織培養学会 第88回大会
  • Place of Presentation: 広島市
  • Year and Date: 2015-05-26
• [Presentation] スキルス胃癌膜タンパク質の大規模プロテオーム解析 (2015)
  • Author(s): 村岡賢, 久米秀明, 橋口一成, 足立淳, 朝長毅, 松原久裕, 木根原匡希, 嶋本 顕, 田原栄俊
  • Organizer: 日本組織培養学会 第88回大会
  • Place of Presentation: 広島市
  • Year and Date: 2015-05-26
• [Presentation] 細胞老化を誘導する microRNA の網羅的スクリーニングとその機能解析 (2015)
  • Author(s): 山本 佑樹, 福永早央里, 高木翔太, 木根原匡希, 村岡賢, 塩谷文章, 嶋本顕, 田原栄俊
  • Organizer: 日本組織培養学会 第88回大会
  • Place of Presentation: 広島市
  • Year and Date: 2015-05-26
• [Presentation] 老化関連 microRNA による膵臓がん抑制メカニズムの解析 (2015)
  • Author(s): 福永 早央里, 山本 佑樹, 木根原 匡希, 村岡 賢, 嶋本 顕, 田原 栄俊
  • Organizer: 日本組織培養学会 第88回大会
  • Place of Presentation: 広島市
  • Year and Date: 2015-05-26
• [Presentation] ウェルナー症候群iPS細胞の樹立と応用に向けた課題 (2015)
  • Author(s): 嶋本 顕, 田原栄俊
  • Organizer: 日本組織培養学会 第88回大会
  • Place of Presentation: 広島市
  • Year and Date: 2015-05-26
• [Presentation] DNA Damage Response in Human Induced Pluripotent Stem Cells (2015)
  • Author(s): Akira Shimamoto,Harunobu Kagawa,Hidehiko Kawai,Tetsushi Sakuma,Takashi Yamamoto,Bunsyo Shiotani,Hidetoshi Tahara
  • Organizer: 第15回国際放射線研究会議（ICRR2015）
  • Place of Presentation: 京都市
  • Year and Date: 2015-05-25
• [Presentation] "Identification and characterization of invasion-associated EV proteins by using proteomics analysis" (2015)
  • Author(s): Satoshi Muraoka,Hidetoshi Tahara
  • Organizer: International Soclety for Extracellular Vesicies 2015
  • Place of Presentation: WashingtonDC,USA
  • Year and Date: 2015-04-25
  • Int'l Joint Research
• [Presentation] Senescence-associated EVs act as tumor suppressors in the tumor microenvironment (2015)
  • Author(s): Hidetoshi Tahara
  • Organizer: International Soclety for Extracellular Vesicies 2015
  • Place of Presentation: WashingtonDC,USA
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] マイクロRNAを用いたバイオマーカーおよび核酸医薬の開発 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第59回分子病態医学セミナー＆TRCセミナー合同セミナー
  • Place of Presentation: 松山市
  • Year and Date: 2015-04-03
  • Invited
• [Presentation] 健康長寿を達成させる最先端未病検知・疾患早期診断検査 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 医総会WEEK
  • Place of Presentation: 京都市
  • Year and Date: 2015-04-03
  • Invited
• [Presentation] microRNAが切り拓く創薬研究の新展開について (2015)
  • Author(s): 田原 栄俊
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] テロメア・マイクロRNA を用いた疾患の予防と早期診断 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第40回日本脳卒中学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] Circulating miRNAs as liquid biopsy in Alzheimer's disease and pancreatic cancer toward application for the diagnosis. (2015)
  • Author(s): Yu Ninose, Sayaka Okamoto, Ikuko Takeda, Tetsuya Takahashi, Masayasu Matsumoto,
  • Organizer: Extracellular Biomarkers Summit
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-06
• [Presentation] マイクロRNA・細胞外小胞がもたらす脳神経内科の未来 (2015)
  • Author(s): 田原 栄俊
  • Organizer: 第13回脳神経内科ジョイントミーティング
  • Place of Presentation: 広島市
  • Year and Date: 2015-03-03
  • Invited
• [Presentation] アルツハイマー患者における血漿中マイクロRNAの解析と診断への応用 (2014)
  • Author(s): 二瀬 由宇、岡本 沙矢香、竹田育子、高橋 哲也、松本 昌泰、田原 栄俊
  • Organizer: 第37回 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-26
• [Presentation] 老化を調節する microRNA の膵臓がん細胞における機能解析 (2014)
  • Author(s): 福永 早央里, 塩谷 文章, 嶋本 顕, 田原 栄俊
  • Organizer: 第37回 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-26
• [Presentation] microRNA による細胞老化誘導機構の解明 (2014)
  • Author(s): 山本 佑樹, 塩谷 文章, 嶋本 顕, 田原 栄俊
  • Organizer: 第37回 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-26
• [Presentation] 細胞老化を誘導する microRNA の網羅的スクリーニング (2014)
  • Author(s): 山本 佑樹, 塩谷 文章, 嶋本 顕, 田原 栄俊
  • Organizer: 平成 26 年度 がん若手研究者ワークショップ
  • Place of Presentation: 長野県茅野市
  • Year and Date: 2014-09-03 – 2014-09-06
• [Presentation] 細胞老化における細胞外小胞エクソソーム分泌の生物学的意義の探索 (2014)
  • Author(s): 岡田 恵、宗岡 美紗、岡本 沙矢香、二瀬 由宇、塩谷 文章、嶋本 顕、田原 栄俊
  • Organizer: 第６回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-29
• [Presentation] Direct detection of extracellular vesicles and exRNA (2014)
  • Author(s): 田原 栄俊
  • Organizer: 第１回日本細胞外小胞学会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-28
  • Invited
• [Presentation] 老化を調節する microRNA による膵臓がん抑制機構の解析 (2014)
  • Author(s): 福永 早央里、塩谷 文章、嶋本 顕、田原 栄俊
  • Organizer: 第６回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-28
• [Presentation] 細胞老化を誘導する microRNA の網羅的スクリーニング (2014)
  • Author(s): 山本 佑樹, 塩谷 文章, 嶋本 顕, 田原 栄俊
  • Organizer: 第６回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-28
• [Presentation] スペクトル型フローサイトメーター SP6800によるエクソソームの一粒子測定 (2014)
  • Author(s): 品末 聡也、岡田 恵、宗岡 美紗、植田 俊樹、佐古 直紀、新田 尚、田原 栄俊
  • Organizer: 第６回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-28
• [Presentation] アルツハイマー患者における血漿中マイクロRNAの解析と診断への応用 (2014)
  • Author(s): 二瀬 由宇、岡本 沙矢香、竹田育子、高橋 哲也、松本 昌泰、田原 栄俊
  • Organizer: 第６回日本RNAi研究会
  • Place of Presentation: 広島市
  • Year and Date: 2014-08-28
• [Presentation] 小さな巨人「マイクロRNA」によるがん診断およびがん治療戦略 (2014)
  • Author(s): 田原 栄俊
  • Organizer: NPO法人国際医科学研究会第８回フォーラム
  • Place of Presentation: 東京都
  • Year and Date: 2014-06-29
  • Invited
• [Presentation] Senescence-associated exosome, the mechanism of secretion and the function (2014)
  • Author(s): Hidetoshi Tahara
  • Organizer: International Symposium on Extracellular Vesicles for Biomedical Applications,KSEV2014 (Korean Society for ExtracellularVesicles)
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2014-05-29 – 2014-05-30
  • Invited
• [Presentation] テロメア・マイクロＲＮＡの検査が切り開くがん診断・治療 (2014)
  • Author(s): 田原 栄俊
  • Organizer: がんと炎症・代謝研究会 講演会
  • Place of Presentation: 京都市
  • Year and Date: 2014-05-25
  • Invited
• [Presentation] Direct Detection of Exosome by SP6800 Spectral Analyzer (2014)
  • Author(s): 品末 聡也
  • Organizer: CYTO2014
  • Place of Presentation: フロリダ(アメリカ)
  • Year and Date: 2014-05-19
• [Presentation] Biogenesis and biological activity of exosomes in replicative senescent cells. (2014)
  • Author(s): 岡田 恵
  • Organizer: ISEV (INTERNATIONAL SOCIETY FOR EXTRACELLULAR VESICLES) 2014
  • Place of Presentation: ロッテルダム(オランダ）
  • Year and Date: 2014-05-01
• [Book] MicroRNA in Regenerative Medicine (2015)
  • Author(s): Dan Xu, Yeqing Sun, Hidetoshi Tahara
  • Total Pages: 1259
  • Publisher: Elsevier