Indentifiation of nucleic acid aptamers which specifically recognize cancer-derived exosomes and its application for diagnostics
| Project/Area Number |
26640097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Miyagishi Makoto 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (30323538)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 核酸アプタマー / エキソソーム / 癌 / バイオマーカー / miRNA |
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| Outline of Final Research Achievements |
Exosomes are cell-secreting lipid vesicles with a diameter of 30-100 nm, which include information (microRNAs and mRNAs) related to their cells/tissues of origin. It is thus to be expected its application to disease diagnostics for cancers and various diseases. In the present study, we attempted to identify aptamers which could distinguish exosomes derived from different cancer cell lines (lung cancer cells, colon cancer cells, and breast cancer cells) by means of counter SELEX method. However we failed in several attempts to obtain aptamers using the conventional nitrocellulose filter selection method. Therefore, to improve efficiency, we utilezed anti-CD68-magnetic beads to capture exosomes as the selection procedure. As a result, we finally identify some aptamers for colon cancer-derived exosomes.
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Report
(3 results)
Research Products
(2 results)
