Study of stress-related disorder monitored by plasma proteomics
Project/Area Number |
26640115
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Genome biology
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Research Institution | Kitasato University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
永井 隆之 北里大学, 感染制御科学府, 准教授 (00172487)
板倉 誠 北里大学, 医学部, 准教授 (30398581)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 血漿 / プロテオミクス / 質量分析 / ストレス障害 / モデルマウス / 血液 / 視床下部 / 海馬 / 安定同位体標識法 / ストレス関連障害 / 安定同位体標識 / 不安様行動 / ペプチドミクス / 高精度比較分析 |
Outline of Final Research Achievements |
Human plasma is by far the most commonly sampled diagnostic biospecimen and a potentially informative resource of extreme importance for characterizing proteomes. However, the existence of high-abundance proteins and the large heterogeneity of protein modification and fragmentation make precise analysis of proteins challenging. In this study, we developed the novel pretreatment method of plasma, which we call PF method, for preseparating plasma proteins. Based on this method, we achieved high-quality comparative analysis of plasma proteins and accurately detected alteration of plasma proteome profiling correlating with stress-related disorder.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Fibrinogen alpha C chain 5.9 kDa fragment (FIC5.9), a biomarker for various pathological conditions, is produced in post-blood collection by fibrinolysis and coagulation factors2016
Author(s)
Kikuchi W, Nishimura M, Kuga T, Tsuchida S, Saito T, Satoh M, Noda K, Kodera Y, Tomonaga T, Nomura F
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Journal Title
Clinical Proteomics
Volume: 13
Issue: 1
Pages: 27-27
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of a novel serum biomarker for pancreatic cancer, C4b-binding protein α-chain (C4BPA) by quantitative proteomic analysis using tandem mass tags.2016
Author(s)
Sogawa K, Takano S, Iida F, Satoh M, Tsuchida S, Kawashima Y, Yoshitomi H, Sanda A, Kodera Y, Takizawa H, Mikata R, Ohtsuka M, Shimizu H, Miyazaki M, Yokosuka O, Nomura F.
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Journal Title
Br J Cancer.
Volume: 115
Issue: 8
Pages: 949-956
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Autoantibodies against vinculin in patients with chronic inflammatory demyelinating polyneuropathy.2015
Author(s)
Beppu M, Sawai S, Satoh M, Mori M, Kazami T, Misawa S, Shibuya K, Ishibashi M, Sogawa K, Kado S, Kodera Y, Nomura F, Kuwabara S.
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Journal Title
J Neuroimmunol.
Volume: 287
Pages: 9-15
DOI
Related Report
Peer Reviewed
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[Journal Article] Moesin is a possible target molecule for cytomegalovirus-related Guillain-Barré syndrome.2014
Author(s)
Sawai S, Satoh M, Mori M, Misawa S, Sogawa K, Kazami T, Ishibashi M, Beppu M, Shibuya K, Ishige T, Sekiguchi Y, Noda K, Sato K, Matsushita K, Kodera Y, Nomura F, Kuwabara S
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Journal Title
Neurology
Volume: 83
Issue: 2
Pages: 113-117
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Discovery of colorectal cancer biomarker candidates by membrane proteomic analysisand subsequent verification using selected reaction monitoring and tissue microarrayanalysis2014
Author(s)
Kume H, Muraoka S, Kuga T, Adachi J, Narumi R, Watanabe S, Kuwano M, Kodera Y, Matsushita K, Fukuoka J, Masuda T, Ishihama Y, Matsubara H, Nomura F, Tomonaga T.
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Journal Title
Mol Cell Proteomics.
Volume: M113
Issue: 6
Pages: 37093-37093
DOI
Related Report
Peer Reviewed
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[Presentation] ヒト血漿中のペプチドの網羅的解析2016
Author(s)
小寺義男,斎藤達也,川島祐介,谷祐至,七里眞義
Organizer
日本プロテオーム学会2016年大会
Place of Presentation
北里大学薬学部白金キャンパス(東京都港区)
Year and Date
2016-07-28
Related Report
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