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Standardization of genome-wide absolute and accurate counting of RNA molecules

Research Project

Project/Area Number 26640119
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SHIROGUCHI Katsuyuki  独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (00454059)

Project Period (FY) 2014-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords高精度定量 / デジタル定量 / バーコーディング / ゲノムワイド / シークエンサ
Outline of Final Research Achievements

We have previously developed the molecular barcoding method which enables one to perform accurate digital counting of the copy number of nucleic acid molecules genome-wide using next generation sequencer. This time, we developed it further, for example, by optimizing the sequence of molecular barcode, and succeeded in obtaining high dynamic range with less cost. These results may push to make our molecular barcoding method world-standard.

Report

(2 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (7 results)

All 2015 2014

All Presentation (7 results) (of which Invited: 4 results)

  • [Presentation] Genome-wide Digital mRNA Counting Using "Molecular Barcoding" and Its Application for Differentiation Study2015

    • Author(s)
      Katsuyuki Shiroguchi
    • Organizer
      BIT’s 5th Annual World Congress of Molecular & Cell Biology-2015
    • Place of Presentation
      Nanjing, China
    • Year and Date
      2015-04-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 100 cell transcriptome analysis: highly accurate absolute digital quantification with single molecule resolution using molecular barcoding2014

    • Author(s)
      城口克之
    • Organizer
      日本免疫学会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Towards next generation system-wide measurements: Highly accurate and absolute quantification of RNA molecules from small amounts of sample by "molecular barcoding"2014

    • Author(s)
      城口克之
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Accurate system-wide gene expression analysis with single molecule resolution by using molecular barcoding2014

    • Author(s)
      Katsuyuki Shiroguchi
    • Organizer
      MicroRNAs & Single Molecule Biology/Genome Editing Europe Symposia-2014
    • Place of Presentation
      Cambridge, UK
    • Year and Date
      2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Absolute genome-wide quantification of gene expression with single molecule resolution using molecular barcoding2014

    • Author(s)
      城口克之
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Highly Reproducible and Sensitive Genome-Wide Digital RNA Counting using Molecular Barcoding2014

    • Author(s)
      Katsuyuki Shiroguchi
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      South Hadley, USA
    • Year and Date
      2014-08-03 – 2014-08-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNA分子数の絶対定量により状態変化を捉える:分子バーコーディング法によるRNAゲノムワイドデジタル計測法の開発とその応用2014

    • Author(s)
      城口克之
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      奈良県新公会堂(奈良県・奈良市)
    • Year and Date
      2014-06-11
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2016-06-03  

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