Standardization of genome-wide absolute and accurate counting of RNA molecules
| Project/Area Number |
26640119
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SHIROGUCHI Katsuyuki 独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (00454059)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 高精度定量 / デジタル定量 / バーコーディング / ゲノムワイド / シークエンサ |
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| Outline of Final Research Achievements |
We have previously developed the molecular barcoding method which enables one to perform accurate digital counting of the copy number of nucleic acid molecules genome-wide using next generation sequencer. This time, we developed it further, for example, by optimizing the sequence of molecular barcode, and succeeded in obtaining high dynamic range with less cost. These results may push to make our molecular barcoding method world-standard.
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Report
(2 results)
Research Products
(7 results)
