Technical development of single-cell multi-omics
| Project/Area Number |
26640120
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Sasagawa Yohei 国立研究開発法人理化学研究所, 情報基盤センター, 上級センター研究員 (10404344)
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| Co-Investigator(Renkei-kenkyūsha) |
Danno Hiroki 国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (20610439)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 1細胞RNA-seq / 1細胞エピゲノムシーケンス / single-cell RNA-seq / cell barcoding / 新生RNA / 1細胞 / マルチオミックス / エピゲノム / トランスクリプト / シングルセル |
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| Outline of Final Research Achievements |
In this study, we tried to develop technology for simultaneous detection of epigenome and transcriptome from one cell. We separated cytosol and nucleus from a single-cell using pipet tip. We tried to use nucleus for detection of epigenome. Moreover, we succeeded to develop single-cell RNA-seq method, which detect non-poly-A RNA including nascent RNA. Moreover, we tried to develop cell-barcoding method using random primer for high-throughput detection of RNA.
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Report
(5 results)
Research Products
(3 results)
