| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
Alzheimer’s disease (AD) is progressive neurodegenerative disorder characterized by two major pathological changes in expansion of senile plaques (SP) and neurofibrillary tangles (NFT) in the brain. Autopsy brains were diagnosed based on the degree of these two pathological findings defined by the Braak stage (SP: 0, A, B, and C, and NFT:0, I, II, III, IV, V and VI). We analyzed non-coding small RNA, especially miRNA, in the exosome as a candidate molecule of propagation of pathological lesion. Total RNA was isolated from three brain regions (entorhinal cortex, temporal cortex and frontal cortex) at several Braak stages. Over 20,000,000 single reads in each sample was determined by the next generation sequencing and statistically analyzed. We found differentially expressed miRNAs in the course of expansion of pathological lesion in each brain region.
|
