Molecular analysis of the differentially expressed miRNA related to progression of Alzheimer's disease
| Project/Area Number |
26640122
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Niigata University |
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Principal Investigator |
Kuwano Ryozo 新潟大学, 脳研究所, フェロー (20111734)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYASHITA Akinori 新潟大学, 脳研究所, 助教 (60323995)
NAKAYA Akihiro 新潟大学, 研究推進機構, 准教授 (60301149)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | アルツハイマー病 / miRNA / 凍結ヒト脳 / ゲノム / 凍結脳 / マイクロRNA / 遺伝子発現 / 脳組織 / RNA / 低分子RNA |
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| Outline of Final Research Achievements |
Alzheimer’s disease (AD) is progressive neurodegenerative disorder characterized by two major pathological changes in expansion of senile plaques (SP) and neurofibrillary tangles (NFT) in the brain. Autopsy brains were diagnosed based on the degree of these two pathological findings defined by the Braak stage (SP: 0, A, B, and C, and NFT:0, I, II, III, IV, V and VI). We analyzed non-coding small RNA, especially miRNA, in the exosome as a candidate molecule of propagation of pathological lesion. Total RNA was isolated from three brain regions (entorhinal cortex, temporal cortex and frontal cortex) at several Braak stages. Over 20,000,000 single reads in each sample was determined by the next generation sequencing and statistically analyzed. We found differentially expressed miRNAs in the course of expansion of pathological lesion in each brain region.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] Autopsy confirmed Alzheimer’s disease, especially early onset and apolipoprotein E4 negative male has the variant in the ATP-binding cassette transporter (ABCA7)2015
Author(s)
H.Akatsu, M.Tomioka, T.Kanematsu, Y.Toda, H.Yamagata, S.amamoto, H.Ohara, T.Ikeuchi, R.Kuwano, Y.Hashizume, K.Ueda
Organizer
International Association of Gerontology and Geriatrics
Place of Presentation
タイ、チェンマイ
Year and Date
2015-10-19
Related Report
Int'l Joint Research
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